Method and composition for the treatment, prevention, and diagnosis of cancer containing or derived from cancer stem cells
The invention provides a method and composition for the treatment, prevention, and diagnosis of cancer containing or derived from cancer stem cells.
1. A method of treating a cancer containing or derived from cancer stem cells comprising a step of administering an siRNA-carrier complex comprising an siRNA against ribophorin II (RPN2) and a transport carrier to a subject in need thereof, wherein the cancer stem cells have a mutated p53 gene, and wherein the siRNA interferes with RPN2 gene expression in an in vitro assay.
2. The method of claim 1 , wherein the transport carrier comprises the peptide AAAAAAK (SEQ ID NO: 26).
3. The method of claim 1 , wherein the siRNA-carrier complex is administered locally.
4. The method of claim 1 , wherein the siRNA-carrier complex is administered systemically.
5. The method of claim 1 , wherein a double-stranded region of the siRNA is 19 bases in length.
6. The method of claim 1 , wherein a double-stranded region of the siRNA comprises position of 1,194 of the RPN2 gene sequence (SEQ ID NO: 1).
7. The method of claim 1 , wherein a double-stranded region of the siRNA corresponds to at least position 1,212 of the RPN2 gene sequence (SEQ ID NO: 1).
8. The method of claim 1 , wherein the siRNA corresponds to positions comprises 1,194-1,212 of the RPN2 gene sequence (SEQ ID NO: 1).
9. The method of claim 1 , wherein the siRNA comprises RNA comprises SEQ ID NO: 2.
10. The method of claim 1 , wherein the siRNA comprises RNA comprising SEQ ID NO: 3.
11. The method of claim 1 , further comprising administering a substance for introducing a nucleic acid.
12. The method of claim 11 , wherein the substance for introducing a nucleic acid is liposome.