Technology for preparation of macromolecular microspheres
Microspheres are produced by contacting an aqueous solution of a protein or other macromolecule with an organic solvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals of defined dimensions.
1. Microparticles comprising a polypeptide comprising the amino acid of SEQ ID NO:17 prepared by a method comprising:
a) adding a counterion selected from the group consisting of sodium citrate, sodium sulfate, and potassium sulfate to an aqueous solution comprising a polypeptide comprising the amino acid sequence of SEQ ID NO:17, whereby an aqueous composition is formed;
b) adding isopropanol to the aqueous composition of (a) whereby a second aqueous composition is formed; and
c) cooling the second aqueous composition of (b) at a rate of 0.1° C./min to 10° C./min to a temperature between 4° C. and −45° C., whereby a composition containing polypeptide microparticles comprising the polypeptide is formed.
2. The microparticles of claim 1 , wherein the counterion is sodium citrate.
3. The microparticles of claim 1 , wherein the method of making further comprises lyophilizing the composition containing polypeptide microparticles to obtain a dry powder.
4. The microparticles of claim 1 , wherein the diameter of the microparticles is between 0.5 μm and 10 μm.
5. The composition of claim 4 , wherein the diameter of the microparticles is between 0.5 μm or and 5.0 μm.
6. The composition of claim 1 , wherein the counterion is sodium sulfate.
7. The composition of claim 1 , wherein the counterion is potassium sulfate.
8. A composition comprising microparticles of a polypeptide comprising the amino acid sequence of SEQ ID NO:17 and sodium sulfate, wherein the microparticles have a diameter.
9. The microparticles of claim 8 , wherein the diameter of the microparticles is between 0.5 μm and 10 μm.
10. The composition of claim 9 , wherein the diameter of the microparticles is between 0.5 μm or and 5.0 μm.