Fungal endophytes for improved crop yields and protection from pests
The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.
1. A method for improving drought resistance in a cotton plant, the method comprising:
contacting a cotton seed with a fluid seed coating comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one Chaetomium species wherein the contacted seed contains at least 10 CFU up to 10,000 CFU of the facultative fungal endophyte coated onto the surface of the seed, wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to improve drought resistance of a cotton plant grown from the contacted seed compared to a reference cotton plant grown from a reference seed.
2. The method of claim 1 , wherein the fluid seed coating contains at least 100 (10^2) spores/ml or 100,000 (10^4) spores/g dry weight of the facultative fungal endophytes.
3. The method of claim 1 , wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to increase biomass.
4. The method of claim 1 , wherein the facultative fungal endophytes are species Chaetomium globosum.
5. The method of claim 4 , wherein the cotton plant is variety LA122.
6. The method of claim 1 , wherein the cotton plant is variety LA122.
7. The method of claim 1 , wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to reduce yield loss.
8. The method of claim 7 , wherein yield loss is reduced by at least 5%.
9. The method of claim 7 , wherein the reduction in yield loss results in a yield increase of at least 5%.
10. The method of claim 1 , wherein enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt as compared to a seedling grown from a reference seed.
11. The method of claim 1 , wherein the seed is surface sterilized before contacting the seed with the fluid seed coating.
12. The method of claim 1 , wherein the contacted seed contains at least 100 CFU, at least 300 CFU, at least 1,000 CFU, at least 3,000 CFU, or at least 10,000 CFU of the facultative fungal endophytes coated onto the surface of the seed.