Cell preparations for extemporaneous use, useful for healing and rejuvenation in vivo
The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell combinations preparations for use in tissue regeneration and bone regeneration and pain reduction.
1. A medical separator system for preparation of a platelet concentrate, comprising:
a tube adapted to be centrifuged for a length of time and containing only two additives, wherein said two additives are:
a thixotropic gel adapted to separate blood components in whole blood to provide a platelet concentrate containing less than or equal to 1% hematocrit and a pellet containing more than or equal to 99% hematocrit by forming a barrier between plasma and erythrocytes; and
an anticoagulant being adapted to at least reduce coagulation of said whole blood.
2. The medical separator system of claim 1 , wherein said thixotropic gel is adapted to separate said blood components in said whole blood to provide said platelet concentrate containing 2 or more times a normal level of platelets and growth factors compared to said whole blood.
3. The medical separator system of claim 2 , wherein said thixotropic gel is adapted to separate said blood components in said whole blood to provide said platelet concentrate with a platelet yield of 95%±5%.
4. A kit comprising:
the medical separator system of claim 3 ; and
an applicator device for applying said platelet concentrate to a patient for medical, therapeutic, or skincare application.
5. The medical separator system of claim 3 , wherein said tube contains between 1 mL and 3 mL of said thixotropic gel.
6. The medical separator system of claim 1 , wherein said anticoagulant is either a sodium citrate solution or anhydrous sodium citrate.
7. The medical separator system of claim 6 , wherein said anticoagulant is a sodium citrate solution at 0.1 M.
8. The medical separator system of claim 7 , wherein said tube contains 1 mL of said anticoagulant.
9. The medical separator system of claim 6 , wherein a concentration of said anticoagulant is 3.5 mg/mL.
10. A medical separator system for preparation of a platelet concentrate, comprising
a tube adapted to be centrifuged for a length of time and containing only two additives, wherein said two additives are:
a thixotropic gel adapted to separate blood components in whole blood to provide a platelet concentrate containing 2 or more times a normal level of platelets and growth factors compared to said whole blood and a pellet containing more than or equal to 99% hematocrit by forming a barrier between plasma and erythrocytes; and
an anticoagulant being adapted to at least reduce coagulation of said whole blood.
11. The medical separator system of claim 10 , wherein said thixotropic gel is adapted to separate said blood components in said whole blood to provide said platelet concentrate with a platelet yield of 95%±5%.
12. A kit comprising:
the medical separator system of claim 11 ; and
an applicator device for applying said platelet concentrate to a patient for medical, therapeutic, or skincare application.
13. The medical separator system of claim 11 , wherein said tube contains between 1 mL and 3 mL of said thixotropic gel.
14. The medical separator system of claim 10 , wherein said anticoagulant is either a sodium citrate solution or anhydrous sodium citrate.
15. The medical separator system of claim 14 , wherein said anticoagulant is a sodium citrate solution at 0.1 M.
16. The medical separator system of claim 14 , wherein said tube contains 1 mL of said anticoagulant.
17. The medical separator system of claim 14 , wherein a concentration of said anticoagulant is 3.5 mg/mL.
18. A medical separator system for preparation of a platelet concentrate, comprising:
a tube adapted to be centrifuged for a length of time and containing only two additives, wherein said two additives are:
a thixotropic gel adapted to separate blood components in whole blood to provide a platelet concentrate having a platelet yield of 95%±5% by forming a barrier between plasma and erythrocytes; and
an anticoagulant being adapted to at least reduce coagulation of said whole blood.
19. The medical separator system of claim 18 , wherein said tube contains between 1 mL and 3 mL of said thixotropic gel.
20. The medical separator system of claim 19 , wherein said anticoagulant is either a sodium citrate solution or anhydrous sodium citrate.
21. The medical separator system of claim 20 , wherein said anticoagulant is a sodium citrate solution at 0.1 M.
22. The medical separator system of claim 20 , wherein a concentration of said anticoagulant is 3.5 mg/mL.
23. A medical separator system for preparation of a blood concentrate, comprising:
a tube adapted to be centrifuged for a length of time and containing only two additives, wherein said two additives are:
a thixotropic gel adapted to separate blood components in blood to provide a blood concentrate containing less than or equal to 1% hematocrit and a platelet yield of 95%±5% by forming a barrier between plasma and erythrocytes; and
an anticoagulant being adapted to at least reduce coagulation of said blood;
wherein said tube contains between 1 mL and 3 mL of said thixotropic gel.
24. The medical separator system of claim 23 , wherein said blood and said blood concentrate are one of:
whole blood and a platelet concentrate, respectively; or
bone marrow and a bone marrow concentrate, respectively.