Recombinant
Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
1. A recombinant E. coli cell comprising: at least one exogenous gene encoding a thioesterase, an exogenous gene encoding an acyl-CoA synthase, and at least one exogenous gene encoding an alcohol forming-CoA reductase, an acyl-CoA reductase, an ester synthase or an alcohol dehydrogenase, wherein the recombinant E. coli cell produces increased concentrations of fatty acid derivatives when grown under conditions sufficient to express the exogenous genes in a medium comprising a carbon source compared to a non-recombinant E. coli cell.
2. The recombinant E. coli cell of claim 1 , wherein the at least one exogenous gene is over-expressed.
3. The recombinant host E. coli cell of claim 1 , wherein the at least one thioesterase gene encodes a thioesterase having EC number: EC 3.1.2.- or EC 3.1.1.5 or EC 3.1.2.14.
4. The recombinant E. coli cell of claim 1 , wherein the least one exogenous gene selected from the group consisting of an exogenous an acyl-CoA reductase gene, an exogenous alcohol dehydrogenase gene and an exogenous fatty alcohol forming acyl-CoA reductase gene is: an exogenous an acylCoA reductase gene.