Peptide array quality control
View Patent ↗The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.
1. A method for determining quality and fidelity of an in situ synthesized peptide array configured for use in an immunosignature assay, comprising:
obtaining a first binding pattern of the in situ synthesized peptide array, wherein the first binding pattern is obtained from binding with a first sample, the first sample comprising a biological sample from a subject, wherein the in situ synthesized peptide array comprises at least 100 compounds;
obtaining a second binding pattern of an in situ synthesized reference peptide array;
comparing the first binding pattern with the second binding pattern and calculating a correlation value, wherein the comparing comprises a direct peptide-by-peptide comparison between the first and the second binding patterns to evaluate a number of peptides expressing different patterns and a comparison between a binding profile of the first binding pattern and a binding profile of the second binding pattern to evaluate a level of similarity between the binding profiles, wherein each of the binding profiles comprises a distribution of signals measured from a population of at least 2 arrayed compounds; and
thereby establishing synthesis quality and fidelity of the in situ synthesized peptide array, wherein a correlation value of less than a threshold level indicates a quality of an immunosignature assay using the in situ synthesized peptide array.
2. The method of claim 1 , wherein the correlation value is obtained by Kolmogorov-Smirnov test, Pearson Correlation or Pearson Squared.
3. The method of claim 1 , wherein the threshold level is defined as a percentage of the correlation coefficient between assays.
4. The method of claim 1 , wherein the peptide array is a random or partially random peptide array.
5. The method of claim 1 , wherein the first binding pattern is obtained from a subset of peptides on the peptide array, and the second binding pattern is obtained from a corresponding subset of peptides on the peptide reference array.
6. The method of claim 1 , wherein the first binding pattern is obtained from binding with the first sample, the second binding pattern is obtained from binding with a second sample, wherein the first sample and the second sample are from a same species.
7. The method of claim 6 , wherein the first sample and the second sample are not from the same subject.
8. The method of claim 6 , wherein the species is human being.
9. The method of claim 6 , wherein the first sample and the second sample are acquired from a blood sample.
10. The method of claim 1 , wherein the biological sample is a blood sample, a serum sample, a plasma sample, a urine sample, a sputum sample, a mucosal sample, a peritoneal fluid sample, a tissue sample, an exudate or an effusion.
11. A method for determining quality and fidelity of an in situ synthesized peptide array configured for use in an immunosignature assay, comprising:
providing an array of peptides, wherein the peptides are synthesized in situ in a stepwise manner on the surface of an array, wherein the in situ synthesized peptide array comprises at least 100 compounds;
incubating a biological sample on the peptide array;
obtaining a first binding pattern of the biological sample binding to the in situ synthesized peptide array;
obtaining a second binding pattern of an in situ synthesized reference peptide array;
comparing the first binding pattern with the second binding pattern and calculating a correlation value, wherein the comparing comprises a direct peptide-by-peptide comparison between the first and the second binding patterns to evaluate a number of peptides expressing different patterns and a comparison between a binding profile of the first binding pattern and a binding profile of the second binding pattern to evaluate a level of similarity between the binding profiles, wherein each of the binding profiles comprises a distribution of signals measured from a population of at least 2 arrayed compounds; and
thereby establishing synthesis quality and fidelity of the in situ synthesized peptide array, wherein a correlation value of less than a threshold level indicates a quality of an immunosignature assay using the in situ synthesized peptide array.
12. The method of claim 11 , wherein the correlation value is obtained by Kolmogorov-Smirnov test, Pearson Correlation or Pearson Squared.
13. The method of claim 11 , wherein the threshold level is defined as a percentage of the correlation coefficient between assays.
14. The method of claim 11 , wherein the peptide array is a random or partially random peptide array.
15. The method of claim 11 , wherein the first binding pattern is obtained from a subset of peptides on the peptide array, and the second binding pattern is obtained from a corresponding subset of peptides on the peptide reference array.
16. The method of claim 11 , wherein the first binding pattern is obtained from binding with the first sample, the second binding pattern is obtained from binding with a second sample, wherein the first sample and the second sample are from a same species.
17. The method of claim 16 , wherein the first sample and the second sample are not from the same subject.
18. The method of claim 16 , wherein the species is human being.
19. The method of claim 16 , wherein the first sample and the second sample are acquired from a blood sample.
20. The method of claim 1 , wherein the biological sample is a blood sample, a serum sample, a plasma sample, a urine sample, a sputum sample, a mucosal sample, a peritoneal fluid sample, a tissue sample, an exudate or an effusion.