IP Library › Granted Patent US 12,270,035
Granted Patent B2
US 12,270,035 · App. 16/092,862 · Granted Apr 8, 2025

Florigen pathway toolkit

Inventors: Zachary Lippman (North Bellmore, NY); Sebastian Soyk (Cold Spring Harbor, NY); Soon-Ju Park (Cold Spring Harbor, NY)
Assignee: Cold Spring Harbor Laboratory
C12N15/8213C12N9/22C12N15/827A01H6/825C12N2310/20
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Quick Facts
Patent No.
US 12,270,035
App. No.
16/092,862
Granted
Apr 8, 2025
Kind
B2
Abstract

Described herein is a florigen pathway toolkit that comprises mutations in genes in the florigen pathway in Solanaceae, such as mutations in genes in the florigen pathway in tomatoes, that are useful to quantitatively modulate or adjust the balance of opposing flowering signals to customize and improve shoot architecture and flowering (flowering time) for tomato breeding, particularly for fresh market tomato breeding.

Claims (46)

1. An sp5g sp double mutant tomato plant comprising:

an sp5g mutant gene in an sp mutant background,

wherein the sp5g mutant gene comprises the coding sequence of SEQ ID NO: 15 having a null mutation in exon 1 (SEQ ID NO: 1),

wherein the sp mutant background is a tomato plant comprising a mutant sp gene comprising

(i) a sequence having at least 99% identity to SEQ ID NO: 8 or

(ii) a sequence comprising SEQ ID NO: 14,

wherein the sp5g sp double mutant tomato plant flowers earlier than a corresponding sp mutant tomato plant that does not comprise the sp5g mutant gene, as measured with reference to the number of leaves produced prior to appearance of first inflorescence.

2. The tomato plant of claim 1 , wherein the mutation in exon 1 comprises a missense mutation, a nonsense mutation, an insertion, a deletion.

3. The tomato plant of claim 2 , wherein the insertion produces an sp5g mutant gene comprising a frameshift or the deletion produces an sp5g mutant gene comprising a frameshift.

4. The tomato plant of claim 1 , which is homozygous for the sp5g mutant gene.

5. The tomato plant of claim 4 , wherein the tomato plant is homozygous for a clustered regularly interspaced short palindromic repeat (CRISPR) engineered sp5g mutant gene.

6. The tomato plant of claim 1 , in which the sp5g sp double mutant tomato plant has a sympodial index that is the same as the sympodial index of the corresponding sp mutant tomato plant that does not comprise the sp5g mutant gene.

7. The tomato plant of claim 1 , which is a Roma tomato plant, a large-fruited inbred tomato plant or a small-fruited inbred tomato plant.

8. A Solanum lycopersicum tomato plant homozygous for an sp5g mutant gene,

wherein the sp5g mutant gene comprises a null mutation in exon 1 (SEQ ID NO: 1),

wherein the tomato plant flowers earlier than a corresponding sibling Solanum lycopersicum tomato plant that does not comprise the sp5g mutant gene, as measured with reference to the number of leaves produced prior to appearance of the first inflorescence.

9. The Solanum lycopersicum tomato plant of claim 8 , wherein the sp5g mutant gene is a CRISPR-induced heritable allele.

10. The Solanum lycopersicum tomato plant of claim 9 , wherein the sp5g mutant gene is a CRISPR-induced heritable allele comprising a sequence having at least 99% identity to SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7.

11. The Solanum lycopersicum tomato plant of claim 8 , in which the tomato plant has a sympodial index that is the same as the sympodial index of a corresponding tomato plant that does not comprise the sp5g mutant gene.

12. The Solanum lycopersicum tomato plant of claim 8 , which is a Roma tomato plant, a large-fruited inbred tomato plant, or a small-fruited inbred tomato plant.

13. A method of reducing time to production of first inflorescence in a tomato plant, as measured with reference to the number of leaves produced prior to appearance of the first inflorescence, comprising:

producing an sp5g sp double mutant tomato plant, an sp5g sp double mutant tomato seed or an sp5g sp double mutant tomato plant part that is homozygous for an sp5g mutant gene in an sp background and maintaining the sp5g sp double mutant tomato plant, the sp5g sp double mutant tomato seed or the sp5g sp double mutant tomato plant part under conditions under which the sp5g sp double mutant tomato plant, the sp5g sp double mutant tomato seed or the sp5g sp double mutant tomato plant part grows,

wherein the sp5g mutant gene comprises the coding sequence of SEQ ID NO: 15 having a null mutation in exon 1 (SEQ ID NO: 1),

wherein the sp mutant background is a tomato plant comprising a mutant sp gene comprising

(i) a sequence having at least 99% identity to SEQ ID NO: 8 or

(ii) a sequence comprising SEQ ID NO: 14.

14. The method of claim 13 , wherein the sp5g mutant gene is a CRISPR-induced heritable allele.

15. The method of claim 13 , wherein the sp5g mutant gene is a CRISPR-induced heritable allele comprising a sequence having at least 99% identity to comprising a sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7.

16. The method of claim 13 , in which the mutant tomato plant that is homozygous for the sp5g mutant gene has a sympodial index that is the same as the sympodial index of a corresponding tomato plant that is not homozygous for the sp5g mutant gene.

17. The method of claim 13 , in which the mutant tomato plant homozygous for the sp5g mutant gene is a Roma tomato plant, a large-fruited inbred tomato plant or a small-fruited inbred tomato plant.

18. The Solanum lycopersicum tomato plant of claim 8 , wherein the sp5g mutant gene encodes an SP5G protein comprising a mutation in the PEBP domain sequence of SEQ ID NO: 16.

19. The tomato plant of claim 1 , further comprising a mutant suppressor of sp (ssp) gene; a mutant single flower truss (sft) gene; or a mutant suppressor of sp (ssp) gene and a mutant single flower truss (sft) gene,

wherein the mutant ssp gene comprises a mutation in an SSP gene comprising a coding sequence of SEQ ID NO: 9,

wherein the mutant sft gene comprises a mutation in an SFT gene comprising a coding sequence of SEQ ID NO: 13.

20. The tomato plant of claim 19 , wherein the mutant ssp gene is heterozygous or the mutant sft gene is heterozygous.

21. The tomato plant of claim 1 , wherein the sp5g mutant gene is a CRISPR engineered sp5g mutant gene.

22. The tomato plant of claim 5 , wherein the CRISPR engineered sp5g mutant gene is CR-sp5g allele a1 comprising a sequence of SEQ ID NO: 6 or CR-sp5g allele a2 comprising a sequence of SEQ ID NO: 7.

23. The tomato plant of claim 19 , wherein the mutant ssp gene is ssp-2129 comprising a sequence of SEQ ID NO: 10 or ssp-610 comprising a sequence of SEQ ID NO: 11.

24. An early-yielding double determinate tomato plant that comprises an sp5g mutant gene in an sp mutant background,

wherein the double determinate tomato plant flowers earlier than the corresponding sp mutant tomato plant that does not comprise an sp5g mutant gene, as measured with reference to the number of leaves produced prior to appearance of first inflorescence,

wherein the sp5g mutant gene comprises a mutation of SP5G in an exon that produces a null sp5g mutant; a mutation in a region of SP5G that encodes a conserved SP5G sequence that produces a null sp5g mutant; or a mutation in a region of SP5G that encodes a specific domain that produces a null sp5g mutant,

wherein SP5G comprises the coding sequence of SEQ ID NO: 15, and

wherein the sp mutant background is a tomato plant comprising a mutant (sp) gene comprising

(i) a sequence having at least 99% identity to SEQ ID NO: 8 or

(ii) a sequence comprising SEQ ID NO: 14.

25. The tomato plant of claim 19 , wherein the mutant sft gene comprises a sequence of SEQ ID NO: 13.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 11, 2020
From: LIPPMAN, ZACHARY; SOYK, SEBASTIAN; PARK, SOON-JU
To: COLD SPRING HARBOR LABORATORY
Reel/Frame 054704/0406 →
CONFIRMATORY LICENSE Recorded May 15, 2020
From: COLD SPRING HARBOR LABORATORY
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 052679/0401 →
Continuity (2)
Provisional Application 62321178 · Apr 11, 2016
Related Publication 20200299705A1 · Sep 24, 2020
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