Polypeptides having xylanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A process for degrading a cellulosic material or a xylan-containing material, comprising: contacting the cellulosic material or the xylan-containing material with one or more enzymes comprising an isolated polypeptide having xylanase activity to thereby degrade the cellulosic or the xylan-containing material, wherein said isolated polypeptide having xylanase activity is selected from the group consisting of
(a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4; and
(b) a fragment of the sequence of amino acids 17 to 389 of SEQ ID NO: 4, wherein the fragment has xylanase activity.
2. The process of claim 1 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
3. The process of claim 1 , wherein the amino acid sequence of the polypeptide has at least 85% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
4. The process of claim 1 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
5. The process of claim 1 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
6. The process of claim 1 , wherein the amino acid sequence of the polypeptide has at least 97% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
7. The process of claim 1 , wherein the amino acid sequence of the polypeptide comprises the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
8. The process of claim 1 , wherein the polypeptide is a variant of the sequence of amino acids 17 to 389 of SEQ ID NO: 4 comprising an amino acid substitution, deletion and/or insertion at one or more positions of the sequence of amino acids 17 to 389 of SEQ ID NO: 4 and wherein the amino acid sequence of the variant has at least 95% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
9. The process of claim 1 , wherein the polypeptide is a fragment of SEQ ID NO: 4, wherein the fragment has xylanase activity.
10. The process of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under very high stringency conditions with the complement of the sequence of nucleotides 49 to 1705 of SEQ ID NO: 3, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
11. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or xylan-containing material with one or more enzymes comprising an isolated polypeptide having xylanase activity to produce a saccharified cellulosic material or a saccharified xylan-containing material, wherein said isolated polypeptide having xylanase activity is selected from the group consisting of (i) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4; and (ii) a fragment of the sequence of amino acids 17 to 389 of SEQ ID NO: 4, wherein the fragment has xylanase activity; and
(b) fermenting the saccharified cellulosic material or the saccharified xylan-containing material with one or more fermenting microorganisms to produce the fermentation product.
12. The process of claim 11 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
13. The process of claim 11 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
14. The process of claim 11 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
15. The process of claim 11 , wherein the amino acid sequence of the polypeptide has at least 97% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
16. The process of claim 11 , further comprising recovering the fermentation product from the fermentation.
17. A process for degrading a cellulosic material or a xylan-containing material, comprising: contacting the cellulosic material or the xylan-containing material with one or more enzymes comprising an isolated polypeptide having xylanase activity, wherein the polypeptide comprises an amino acid sequence having at least 90% sequence identity to the sequence of amino acids 17 to 326 of SEQ ID NO: 4.
18. The process of claim 17 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
19. The process of claim 17 , wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 17 to 326 of SEQ ID NO: 4.
20. The process of claim 17 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the sequence of amino acids 17 to 326 of SEQ ID NO: 4.
21. The process of claim 17 , wherein the polypeptide comprises the sequence of amino acids 17 to 326 of SEQ ID NO: 4.
22. The process of claim 17 , wherein the polypeptide further comprises a cellulose binding domain.
23. A recombinant host cell transformed with a polynucleotide encoding a polypeptide having xylanase activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the host cell, wherein the polypeptide is heterologous to the host cell, and wherein the polypeptide having xylanase activity is selected from the group consisting of
(a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4; and
(b) a fragment of the sequence of amino acids 17 to 389 of SEQ ID NO: 4, wherein the fragment has xylanase activity.
24. A method of producing a polypeptide having xylanase activity, comprising cultivating the recombinant host cell of claim 23 under conditions conducive for production of the polypeptide.
25. The method of claim 24 , further comprising recovering the polypeptide.
26. The process of claim 1 , wherein the amino acid sequence of the polypeptide comprises the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
27. The recombinant host cell of claim 23 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
28. The recombinant host cell of claim 23 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
29. The recombinant host cell of claim 23 , wherein the amino acid sequence of the polypeptide has at least 97% sequence identity to the sequence of amino acids 17 to 389 of SEQ ID NO: 4.
30. The recombinant host cell of claim 23 , wherein the amino acid sequence of the polypeptide comprises the sequence of amino acids 17 to 389 of SEQ ID NO: 4.