Acoustic cell separation techniques and processes
Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.
1. A method for separating biomaterial, comprising:
applying a functionalized material to a plurality of beads that are free of ferro-magnetic material;
suspending the beads in a fluid to obtain a bead suspension;
exposing the bead suspension to biomaterial with an affinity for the functionalized material to permit the biomaterial to bind to the beads; and
flowing the bead suspension in a chamber to an acoustic field generated in the chamber that traps the beads in the chamber against fluid flow.
2. The method of claim 1 , further comprising:
employing a column to house the bead suspension, such that a fluidized bed is formed with the bead suspension in the column; and
retaining the beads in the column against fluid flow with the acoustic field.
3. The method of claim 1 , further comprising:
generating an angled acoustic wave at an oblique angle to a direction of fluid flow of the bead suspension; and
deflecting the beads in the bead suspension away from a direction of fluid flow to permit separation of the beads from the fluid.
4. The method of claim 1 , wherein the beads comprise acoustic beads.
5. The method of claim 1 , wherein at least some of the plurality of beads are composed with an affinity for one or more of CD3, CD4 or CD8 receptors.
6. The method of claim 1 , wherein the beads are one or more of biocompatible, biodegradable, or inert.
7. The method of claim 1 , wherein the beads comprise one or more of agarose or a perfluorocarbon.
8. The method of claim 1 , wherein the functionalized material is a streptavidin conjugate or a biotin conjugate.
9. The method of claim 1 , wherein the beads comprise a liquid core and a lipid shell.
10. The method of claim 1 , wherein the functionalized material includes affine molecules that are one or more of antibodies, aptamers or oligonucleotides.
11. The method of claim 1 , wherein the functionalized material comprises a ligand.
12. The method of claim 1 , further comprising reversing a binding between the biomaterial and the beads.
13. The method of claim 1 , further comprising:
isolating a first biomaterial and isolating a second biomaterial that is different from the first biomaterial; and
combining the first biomaterial and the second biomaterial in a predetermined ratio.
14. The method of claim 1 , wherein the beads further comprise at least two different sizes of beads, each size being configured with an affinity for a different type of biomaterial.