Reduced gluten grains and compositions thereof
Plants with reduced gluten grains and compositions thereof are disclosed herein.
1. Wheat grain comprising: a wheat prolamin box binding factor (“WPBF”) gene with one or more non-transgenic human induced mutations encoding a WPBF protein comprising an amino acid sequence having 95% or greater identity to SEQ ID NO: 3 or having 95% or greater identity to SEQ ID NO: 6 or having 95% or greater identity to SEQ ID NO: 9, wherein the grain comprises an increase in lysine content as compared to grain from a wild type plant.
2. The wheat grain of claim 1 , wherein the grain further comprises a reduced gluten content as compared to grain from a wild type plant.
3. The wheat grain of claim 1 , wherein the increase in lysine content is at least 20% as compared to grain from a wild type wheat plant.
4. The wheat grain of claim 1 , wherein the grain further comprises a reduction in gliadin content as compared to grain from a wild-type wheat plant.
5. The wheat grain of claim 4 , wherein gliadin content is reduced at least 20% as compared to grain from a wild type wheat plant.
6. A method of altering glutenin content in a wheat plant comprising:
(a) introducing at least one human induced mutations in a WPBF gene in plant material or plant parts from the wheat plant;
(b) growing the plant material or plant parts to produce progeny wheat plants; and
(c) selecting progeny wheat plants that produce wheat grain having one or more non-transgenic human induced alterations in the WPBF gene encoding a WPBF protein comprising an amino acid sequence having 95% or greater identity to SEQ ID NO: 3 or having 95% or greater identity to SEQ ID NO: 6 or having 95% or greater identity to SEQ ID NO: 9, wherein the grain comprises an increase in lysine content as compared to grain from a wild type plant.
7. The method of claim 6 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 2.
8. The method of claim 6 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 5.
9. The method of claim 6 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 8.
10. The method of claim 6 , wherein the grain further comprises a reduced gluten content as compared to grain from a wild type plant.
11. The method of claim 6 , wherein the increase in lysine content is at least 20% as compared to grain from a wild type wheat plant.
12. The method of claim 6 , wherein the grain further comprises a reduction in gliadin content as compared to grain from a wild-type wheat plant.
13. The method of claim 6 , wherein gliadin content is reduced at least 20% as compared to grain from a wild type wheat plant.
14. The method of claim 6 , further comprising harvesting the wheat grain from the selected progeny wheat plants.
15. The wheat grain of claim 1 , wherein the WPBF protein encoded by the WPBF gene of the B genome has a tryptophan to a stop codon substitution at position 70 of SEQ ID NO. 6.