IP Library › Granted Patent US 12,239,126
Granted Patent B2
US 12,239,126 · App. 16/705,966 · Granted Mar 4, 2025

Methods and compositions for preserving tissues and organs

Inventors: Martin L. Yarmush (Newton, MA); Mehmet Toner (Wellesley, MA); Maria-Louisa Izamis (Boston, MA); Timothy Antonie Berendsen (Melrose, MA); Robert Marius Bieganski (Cambridge, MA); Osman Berk Usta (Watertown, MA); Basak Elif Uygun (Newton, MA); Mustafa Korkut Uygun (Newton, MA); Sinem Perk (Chicago, IL)
Assignee: The General Hospital Corporation
A01N1/0226G01N33/5091G01N1/42
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Quick Facts
Patent No.
US 12,239,126
App. No.
16/705,966
Granted
Mar 4, 2025
Kind
B2
Abstract

The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Claims (15)

1. A method for extending the viable preservation of a harvested organ for transplantation, the method comprising:

contacting at least a portion of the harvested organ with a preservation perfusion solution, wherein the preservation perfusion solution comprises (i) at least 3 of the following: insulin, L-glutamine, hydrocortisone, heparin, penicillin, streptomycin sulfate, and adenosine, and (ii) a PPARγ ligand selected from the group consisting of troglitazone and rosiglitazone, or derivatives thereof,

wherein the harvested organ is a liver, pancreas, kidney, spleen, or lung.

2. The method of claim 1 , wherein the preservation perfusion solution does not comprise erythrocytes.

3. The method of claim 1 , wherein contacting at least a portion of the harvested organ with the preservation perfusion solution comprises immersing, partially or completely, the harvested organ with the preservation perfusion solution.

4. The method of claim 1 , wherein contacting at least a portion of the harvested organ with the preservation perfusion solution comprises perfusing the harvested organ with the preservation perfusion solution.

5. The method of claim 1 , wherein the harvested organ has ischemic damage.

6. The method of claim 1 , wherein the harvested organ is cooled and stored at a predetermined sub-zero temperature without freezing.

7. The method of claim 5 , wherein the harvested organ is contacted with a media comprising a supercoolant agent prior to cooling and freezing.

8. The method of claim 7 , wherein the supercoolant agent is selected from the group consisting of: 3-O-methyl-glucose (30 MG), hypothermosol, trehalose, polyvinyl alcohol, polyvinylpyrrolidone, DMSO, polygylcerol, K3Glc (Kaempferol 3-O-β-D-glucopyranoside); K7Glc (Kaempferol 7-O-β-D-glucopyranoside), Q7Glc (Quercetin 7-O-β-D-glucopyranoside), Q3Ga1 (Quercetin 3-O-β-D-galactopyranoside), and polyethylene glycol.

9. The method of claim 8 , wherein the supercoolant agent is 3-O-methyl-glucose (30 MG).

10. A method for extending the viable preservation of a harvested organ, the method comprising:

contacting at least a portion of the harvested organ with a first preservation perfusion solution, wherein the first preservation perfusion solution comprises at least 3 of the following: insulin, L-glutamine, hydrocortisone, heparin, penicillin, streptomycin sulfate, and adenosine; and

contacting at least a portion of the harvested organ with a second preservation solution comprising a PPARγ ligand selected from the group consisting of troglitazone and rosiglitazone, or derivatives thereof,

wherein the harvested organ is a liver, pancreas, kidney, spleen, or lung.

Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2020
From: TONER, MEHMET; USTA, OSMAN BERK
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 052426/0470 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2020
From: PERK, SINEM
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 052426/0558 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2020
From: BIEGANSKI, ROBERT MARIUS
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 052426/0623 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2020
From: YARMUSH, MARTIN L.; IZAMIS, MARIA-LOUISA; BERENDSEN, TIM ANTONIE; UYGUN, BASAK ELIF; UYGUN, MUSTAFA KORKUT
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 052426/0836 →
Continuity (5)
Division 13695459
Provisional Application 61447905 · Mar 1, 2011
Provisional Application 61364186 · Jul 14, 2010
Provisional Application 61330959 · May 4, 2010
Related Publication 20200236924A1 · Jul 30, 2020
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