Analyte sensing biointerface
Disclosed herein is an analyte sensing biointerface that comprises a sensing electrode incorporated within a non-conductive matrix comprising a plurality of passageways extending through the matrix to the sensing electrode. Also disclosed herein are methods of manufacturing a sensing biointerface and methods of detecting an analyte within tissue of a host using an analyte sensing biointerface.
1. A transcutaneous continuous glucose sensor system comprising:
a substantially planar sensor, the sensor comprising:
a first conductive layer associated with a first electrode;
a first non-conductive layer located at least in part over the first conductive layer;
a second conductive layer associated with a second electrode, wherein the second conductive layer is located at least in part over the first non-conductive layer;
a second non-conductive layer located at least in part over the second conductive layer;
a third conductive layer associated with a third electrode, wherein the third conductive layer is located at least in part over the second non-conductive layer; and
a membrane located over at least a portion of a working electrode;
wherein at least one of the first electrode, the second electrode, or the third electrode is the working electrode, and wherein the working electrode is configured to measure a signal indicative of a glucose concentration.
2. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane comprises an enzyme.
3. The transcutaneous continuous glucose sensor system of claim 2 , wherein the membrane further comprises a resistance domain located over the enzyme.
4. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane comprises a bioactive agent.
5. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane is substantially planar.
6. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane covers at least a portion of the first conductive layer and at least a portion of the third conductive layer.
7. The transcutaneous continuous glucose sensor system of claim 1 , wherein at least a portion of the first non-conductive layer is in physical contact with at least a portion of the first conductive layer.
8. The transcutaneous continuous glucose sensor system of claim 1 , further comprising sensor electronics configured to electrically couple to the working electrode.
9. The transcutaneous continuous glucose sensor system of claim 1 , wherein at least a portion of the first non-conductive layer is physically separate from the first conductive layer.
10. The transcutaneous continuous glucose sensor system of claim 1 , further comprising sensor electronics having one or more contacts for operably connecting to the working electrode.
11. A transcutaneous continuous glucose sensor system, comprising:
a substantially planar sensor, the sensor comprising:
a first conductive layer associated with a first electrode, wherein the first electrode is a counter electrode;
a first non-conductive layer located over at least a portion of the first conductive layer;
a second conductive layer associated with a second electrode, wherein the second conductive layer is located over at least a portion of the first non-conductive layer, wherein the second electrode is a working electrode;
a second non-conductive layer located over at least a portion of the second conductive layer;
a third conductive layer associated with a third electrode, wherein the third conductive layer is located over at least a portion of the second non-conductive layer, wherein the third electrode is a reference electrode; and
a membrane located over at least a portion of the working electrode.
12. The transcutaneous continuous glucose sensor system of claim 11 , wherein the membrane comprises an enzyme.
13. The transcutaneous continuous glucose sensor system of claim 12 , wherein the membrane further comprises a resistance domain located over the enzyme.
14. The transcutaneous continuous glucose sensor system of claim 11 , wherein the membrane further comprises a bioactive agent.
15. The transcutaneous continuous glucose sensor system of claim 11 , wherein the membrane is substantially planar.
16. The transcutaneous continuous glucose sensor system of claim 11 , wherein the membrane covers at least a portion of the first conductive layer and at least a portion of the third conductive layer.
17. The transcutaneous continuous glucose sensor system of claim 11 , wherein at least a portion of the first non-conductive layer is in contact with at least a portion of the first conductive layer.
18. The transcutaneous continuous glucose sensor system of claim 11 , further comprising sensor electronics configured to electrically couple to the working electrode.
19. The transcutaneous continuous glucose sensor system of claim 11 , wherein at least a portion of the first non-conductive layer is physically separate from the first conductive layer.
20. The transcutaneous continuous glucose sensor system of claim 11 , further comprising sensor electronics comprising at least one contact for establishing electrical communication with the working electrode.