IP Library › Granted Patent US 12,275,801
Granted Patent B2
US 12,275,801 · App. 16/867,252 · Granted Apr 15, 2025

Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase

Inventors: Stan Gronthos (Adelaide, AU); Andrew Christopher William Zannettino (Highbury, AU); John Paul Simmons (Kew, AU)
Assignee: MESOBLAST, INC.
C07K16/40C12N5/0081C12N5/0663C12N5/163C12Q1/42G01N33/577G01N33/68A61K2035/124C07K2317/76C12N2533/56G01N2333/916
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Quick Facts
Patent No.
US 12,275,801
App. No.
16/867,252
Granted
Apr 15, 2025
Kind
B2
Abstract

The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.

Claims (20)

1. A cell population comprising multipotential cells enriched for TNAP+multipotential cells, wherein at least 4% of the total cell population are TNAP+, STRO-1+multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes and wherein the TNAP+STRO-1+multipotential cells include a TNAP+marker which binds to the STRO-3 antibody produced by the hybridoma cell line deposited under ATCC Accession Number PTA-7282.

2. A method of generating a committed cell population of a specific tissue type, the method comprising culturing a cell population comprising multipotential cells according to claim 1 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the multipotential cells to the specific tissue type.

3. A method for generating or repairing a tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 1 so as to generate or repair the tissue.

4. A composition comprising the cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 1 .

5. A method for generating or repairing a tissue in a subject in need thereof, the method comprising administering to the subject the composition according to claim 4 so as to generate or repair the tissue.

6. The cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 1 , wherein the STRO-1+ cells are STRO-1 bright cells.

7. A composition comprising the cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 6 .

8. A method of generating a committed cell population of a specific tissue type, the method comprising culturing a cell population comprising multipotential cells according to claim 6 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the multipotential cells to the specific tissue type.

9. A method for generating or repairing a tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 6 so as to generate or repair the tissue.

10. The cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 1 , wherein at least 10% of the total cell population are TNAP+, STRO-1+multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes.

11. The cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 10 , wherein the STRO-1+ cells are STRO-1 bright cells.

12. A composition comprising the cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 10 .

13. A method of generating a committed cell population of a specific tissue type, the method comprising culturing a cell population comprising multipotential cells according to claim 10 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the multipotential cells to the specific tissue type.

14. A method for generating or repairing a tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 10 so as to generate or repair the tissue.

15. The cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 10 , wherein at least 20% of the total cell population are TNAP+, STRO-1+multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes.

16. The cell population comprising multipotential cells enriched for TNAP+multipotential cells according to claim 15 , wherein the STRO-1+ cells are STRO-1 bright cells.

17. A composition comprising the cell population comprising multipotential cells enriched for TNAP* multipotential cells according to claim 15 .

18. A method of generating a committed cell population of a specific tissue type, the method comprising culturing a cell population comprising multipotential cells according to claim 15 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the multipotential cells to the specific tissue type.

19. A method for generating or repairing a tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 15 so as to generate or repair the tissue.

20. A method of obtaining a cell population comprising multipotential cells enriched for TNAP+, STRO-1+multipotential cells which comprises contacting the multipotential cells with a STRO-3 antibody produced by the hybridoma cell line deposited under ATCC Accession Number PTA-7282 under binding conditions, recovering the multipotential cells to which the STRO-3 antibody binds, and then separately recovering the multipotential cells to which the STRO-3 antibody bound.

Assignments (4)
RELEASE OF SECURITY INTEREST IN INTELLECTUAL PROPERTY Recorded Jan 2, 2026
From: OAKTREE FUND ADMINISTRATION, LLC, AS AGENT
To: MESOBLAST LIMITED; MESOBLAST UK LIMITED; MESOBLAST, INC. (FORMERLY KNOWN AS ANGIOBLAST, INC.); MESOBLAST INTERNATIONAL SÀRL
Reel/Frame 074174/0183 →
SECURITY INTEREST Recorded Dec 10, 2021
From: MESOBLAST LIMITED ACN 109 431 870; MESOBLAST UK LIMITED; MESOBLAST, INC. (FORMERLY KNOWN AS ANGIOBLAST, INC.); MESOBLAST INTERNATIONAL SÀRL
To: OAKTREE FUND ADMINISTRATION, LLC
Reel/Frame 058957/0447 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 14, 2020
From: GRONTHOS, STAN; ZANNETTINO, ANDREW CHRISTOPHER WILLIAM; SIMMONS, PAUL JOHN
To: MESOBLAST, INC.
Reel/Frame 052667/0167 →
CHANGE OF NAME Recorded May 14, 2020
From: ANGIOBLAST SYSTEMS, INC.
To: MESOBLAST, INC.
Reel/Frame 052672/0900 →
Continuity (7)
Continuation 15651469 · Jul 17, 2017
Continuation 15167934 · May 27, 2016
Division 14621470 · Feb 13, 2015
Division 13736790 · Jan 8, 2013
Continuation 11918593
Provisional Application 60670250 · Apr 12, 2005
Related Publication 20200332023A1 · Oct 22, 2020
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