Method and system to facilitate the growth of desired bacteria in a human's mouth
A method to facilitate the growth of desired bacteria in a human's mouth by using a bioadhesive strip that has an encapsulated feature containing at least one bacteria that has one of a pathogenic or toxic element excised using a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Francisella 1 (Cpf1) system, and in particular, using oral strips that adhere to surfaces in the oral cavity and that include at least one of xylitol, Lachnospira, Veillonella, Faecalibacterium and/or Rothia bacteria.
1. A method to facilitate the growth of desired bacteria in a human's mouth to limit the number of pathogenic bacteria, to maintain oral health and to establish a biofilm in a human subject's mouth, comprising,
after a human subject has cleaned their teeth, killing gram negative bacteria that are associated with periodontitis;
providing to the human subject a bacterial composition comprising beneficial bacteria adapted to form a biofilm, said beneficial bacteria comprising a bacterium of the genus Rothia , and wherein said bacterial composition does not inhibit growth of a bacterium of species Lactobacillus salivarius , and wherein said step of killing comprises using a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Francisella 1 (Cpf1) system.
2. The method as set forth in claim 1 , wherein said beneficial bacteria is provided on an oral strip.
3. The method as set forth in claim 1 , wherein said strip has a first side and a second side, said second side having a bioadhesive that is adapted to bind to a mucosal membrane of the human subject for at least 1 hour while inside the human subject's mouth.
4. The method as set forth in claim 1 , wherein said beneficial bacteria further comprises Lactobacillus reuteri in an amount sufficient to induce oxytocin production.
5. The method as set forth in claim 1 , wherein said gram negative bacteria comprises Treponema denticola.
6. The method as set forth in claim 1 , wherein the bacterial composition is administered orally.
7. The method as set forth in claim 1 , wherein the bacterial composition does not inhibit growth of a second bacterium selected from at least one of the species Streptococcus mutans and Lactobacillus fermentum.
8. The method of claim 1 , wherein the step of administering comprises providing a buccal bioadhesive strip having a first side and a second side, the second side having a bioadhesive that is adapted to bind to a mucosal membrane for at least 1 hour while inside the human subject's mouth, wherein said strip includes at least one encapsulated feature containing said bacterial composition.
9. The method as set forth in claim 1 , wherein the bacterial composition does not inhibit growth of a bacterium of species Lactobacillus fermentum.
10. The method as set forth in claim 1 , wherein the method further comprises administering a prebiotic to the human.
11. The method as set forth in claim 1 , further comprising reducing the population of bacteria that spirochetes depend upon to thrive and grow.
12. The method as set forth in claim 1 , wherein said bacteria comprise P. gingivalis.
13. The method as set forth in claim 1 , further comprising limiting the population of Treponema denticola.
14. A method to facilitate the growth of desired bacteria in a human's mouth to limit the number of pathogenic bacteria, to maintain oral health and to establish a biofilm in a human subject's mouth, comprising,
after a human subject has cleaned their teeth, killing gram negative bacteria that are associated with periodontitis;
providing to the human subject a bacterial composition comprising beneficial bacteria adapted to form a biofilm, said beneficial bacteria comprising a bacterium of the genus Rothia , and wherein said bacterial composition does not inhibit growth of a bacterium of species) Lactobacillus salivarius , and reducing the population of bacteria that spirochetes depend upon to thrive and grow using a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Francisella 1 (Cpf1) system.
15. The method as set forth in claim 14 , wherein said beneficial bacteria is provided on an oral strip.
16. The method as set forth in claim 14 , wherein said strip has a first side and a second side, said second side having a bioadhesive that is adapted to bind to a mucosal membrane of the human subject for at least 1 hour while inside the human subject's mouth.
17. The method as set forth in claim 14 , wherein said beneficial bacteria further comprises Lactobacillus reuteri in an amount sufficient to induce oxytocin production.
18. The method as set forth in claim 14 , wherein said gram negative bacteria comprises Treponema denticola.
19. The method as set forth in claim 14 , wherein the bacterial composition does not inhibit growth of a second bacterium selected from at least one of the species Streptococcus mutans and Lactobacillus fermentum.
20. The method as set forth in claim 14 , wherein the bacterial composition does not inhibit growth of a bacterium of species Lactobacillus salivarius.