Ruminant compositions
The present disclosure provides compositions formulated for ruminants that comprise beneficial microbes. Particularly, the disclosure provides a composition comprising microorganisms with at least 97% sequence identity to any one of SEQ ID NOs: 1-60 and 2045-2107. Such compositions can be used for increasing milk production or improving milk compositional characteristics in ruminants.
1. A method for increasing milk production or improving milk compositional characteristics in a ruminant, the method comprising orally administering to a ruminant an effective amount of a ruminant supplement comprising:
a) a purified microbial population that comprises at least one bacterium with a 16S nucleic acid sequence and/or at least one fungus with an ITS nucleic acid sequence, which is at least 97% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-60 and 2045-2107; and
b) a carrier suitable for ruminant administration, and
wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production or improved milk compositional characteristics, as compared to a ruminant not administered the ruminant supplement.
2. The method according to claim 1 , wherein the ruminant is a cow.
3. The method according to claim 1 , wherein the ruminant supplement is formulated as an encapsulation, tablet, capsule, pill, feed additive, food ingredient, food additive, food preparation, food supplement, consumable solution, consumable spray additive, consumable solid, consumable gel, bolus, drench, or combinations thereof.
4. The method according to claim 1 , wherein the ruminant supplement is encapsulated in a polymer or carbohydrate.
5. The method according to claim 1 , wherein administering comprises: feeding the ruminant supplement to a ruminant.
6. The method according to claim 1 , wherein the purified microbial population is present in the ruminant supplement in an amount of at least 10 2 cells.
7. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 97% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103.
8. The method according to claim 1 , wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 97% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107.
9. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 99% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103.
10. The method according to claim 1 , wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 99% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107.
11. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103.
12. The method according to claim 1 , wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107.
13. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 28.
14. The method according to claim 1 , wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 97% identical to SEQ ID NO: 32.
15. The method according to claim 1 , wherein the purified microbial population comprises a bacteria with a 16S nucleic acid sequence and/or a fungus with an ITS nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-60 and 2045-2107.
16. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence of SEQ ID NO: 28.
17. The method according to claim 1 , wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence of SEQ ID NO: 32.
18. The method according to claim 1 , wherein the purified microbial population comprises a Pichia fungus as deposited at NRRL Y-67249.
19. The method according to claim 1 , wherein the purified microbial population comprises a Clostridium bacterium as deposited at NRRL B-67248.
20. The method according to claim 1 , wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence of SEQ ID NO: 28 and/or a fungi with an ITS nucleic acid sequence of SEQ ID NO: 32.
21. The method according to claim 1 , wherein the purified microbial population comprises a Pichia fungus as deposited at NRRL Y-67249 and/or a Clostridium bacteria as deposited at NRRL B-67248.
22. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production that leads to a measured increase in milk yield.
23. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production and improved milk compositional characteristics that leads to a measured increase in energy-corrected milk.
24. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an improved milk compositional characteristic selected from the group consisting of an increase in milk fat(s), an increase in milk protein(s), an increase of carbohydrates in milk, an increase of vitamins in milk, an increase of minerals in milk, or combinations thereof.
25. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 1% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.
26. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 10% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.
27. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 20% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.
28. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits:
at least one improved phenotypic trait, selected from the group consisting of an improved efficiency in feed utilization, improved digestibility, an increase in polysaccharide and lignin degradation, an increase in fatty acid concentration in the rumen, pH balance in the rumen, a reduction in methane emissions, a reduction in manure production, improved dry matter intake, an improved efficiency of nitrogen utilization, or combinations thereof.
29. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen.
30. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen, wherein a population of microbes present in the rumen before administration of the ruminant supplement increase in abundance after administration of the ruminant supplement.
31. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen, wherein a population of microbes present in the rumen before administration of the ruminant supplement decrease in abundance after administration of the ruminant supplement.
32. The method according to claim 1 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen,
wherein a first population of microbes present in the rumen before administration of the ruminant supplement increase in abundance after administration of the ruminant supplement, and
wherein a second population of microbes present in the rumen before administration of the ruminant supplement decrease in abundance after administration of the ruminant supplement.
33. The method according to claim 1 , wherein the purified microbial population only contains organisms that are members of a group selected from Intestinimonas, Anaerolinea, Pseudobutyrivibrio, Olsenella, Eubacterium, Catenisphaera, Faecalibacterium, Solobacterium, Blautia, Ralsonia, Coprococcus, Casaltella, Anaeroplasma, Acholeplasma, Aminiphilus, Mitsuokella, Alistipes, Sharpea, Oscillibacter, Neocallimastix, Odoribacter, Pichia, Tannerella, Candida, Hydrogenoanaerobacterium, Orpinomyces, Succinivibrio, Sugiyamaella, Ruminobacter, Lachnospira, Caecomyces, Sinimarinibacterium, Tremella, Hydrogenoanaerobacterium, Turicibacter, Clostridium XIVa, Anaerolinea, Saccharofermentans, Butyricicoccus, Olsenella, Papillibacter, Clostridium XIa, Pelotomaculum , Erysipelotrichaceae incertae sedis, Lachnospiracea incertae sedis, Solobacterium, Anaeroplasma, Ralstonia, Clostridium sensu stricto, Eubacterium, Rikenella, Lachnobacterium, Tannerella, Acholeplasma, Howardella, Selenomonas, Butyricimonas, Sharpea, Succinivibrio, Ruminobacter, Candida, Syntrophococcus, Pseudobutyrivibrio, Orpinomyces, Cyllamyces, Saccharomycetales, Phyllosticta, Ascomycota , and Piromyces.
34. The method according to claim 1 , wherein the at least one bacterium or fungus is capable of converting a carbon source into a volatile fatty acid selected from the group consisting of acetate, butyrate, propionate, or combinations thereof.
35. The method according to claim 1 , wherein the at least one bacterium or fungus is capable of degrading a soluble or insoluble carbon source.