Methods, systems, and arrays for biomolecular analysis
Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and methods of assuring a uniformly high quality of a microarray of features that are attached to a surface of the microarray at positionally-defined locations.
1. A method of assuring uniformly high quality of a microarray of features that are attached to a surface of the microarray at positionally-defined locations, comprising steps of:
a) soft-baking the microarray coated with a coupling formulation, the coupling formulation comprising the features;
b) exposing the soft-baked microarray to light under a photomask, the photomask comprising a diffusion pattern and an overlay pattern;
c) hard-baking the exposed microarray; and
d) determining a percent deviation of the diffusion pattern or the overlay pattern of the hard-baked microarray as compared to a standard test diffusion or overlay pattern; and
e) optionally stripping off the coat if the percent deviation of the diffusion pattern or the overlay pattern is greater than 5% and repeating steps (a) through (e).
2. The method of claim 1 , wherein the microarray is soft-baked at a temperature from 75° C. to 115° C.
3. The method of claim 2 , wherein the microarray is soft-baked at 85° C.
4. The method of claim 1 , wherein the microarray is soft-baked from between 60 seconds to 120 seconds.
5. The method of claim 4 , wherein the microarray is soft-baked for 90 seconds.
6. The method of claim 1 , wherein the exposing step comprises a light at 248 nm.
7. The method of claim 1 , wherein the microarray is hard-baked at a temperature from 75° C. to 115° C.
8. The method of claim 7 , wherein the microarray is hard-baked at 85° C.
9. The method of claim 1 , wherein the microarray is hard-baked from between 60 seconds to 120 seconds.
10. The method of claim 9 , wherein the microarray is hard-baked for 90 seconds.
11. The method of claim 1 , wherein the coupling formulation comprises a photoactive compound, a polymer, and a coupling molecule.
12. The method of claim 11 , wherein the photoactive compound comprises a photobase.
13. The method of claim 12 , wherein the coupling molecule comprises an amino acid.
14. The method of claim 11 , wherein the coupling formulation further comprises a coupling reagent and a solvent.