Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.
1. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a first nucleic acid-containing sample and a second nucleic acid-containing sample, wherein the module comprises:
a first lane comprising a first process chamber;
a waste chamber; and
a second lane comprising a second process chamber;
an assembly, wherein the first process chamber and the second process chamber are configured to be received by the assembly, the assembly comprising:
a magnetic assembly configured to apply a magnetic force to the first process chamber and the second process chamber, and
a heater assembly configured to apply heat to the first process chamber and the second process chamber,
wherein, when the first nucleic acid-containing sample and a first plurality of magnetic affinity beads are received in the first process chamber and when the second nucleic acid-containing sample and a second plurality of magnetic affinity beads are received in the second process chamber, the first nucleic acid-containing sample and the first plurality of magnetic affinity beads are configured to be heated within the first process chamber by the heater assembly and wherein the second nucleic acid-containing sample and the second plurality of magnetic affinity beads are configured to be heated within the second process chamber by the heater assembly, and
wherein the magnetic assembly is configured to simultaneously capture or move the first plurality of magnetic affinity beads in the first process chamber and the second plurality of magnetic affinity beads in the second process chamber; and
a liquid dispenser configured to perform suck and dispense operations on the first lane and the second lane, wherein when the first nucleic acid-containing sample is received in the first process chamber and when the second nucleic acid-containing sample is received in the second process chamber, the liquid dispenser is configured to withdraw all or substantially all of a liquid of the first nucleic acid-containing sample from the first process chamber, and all or substantially all of a liquid of the second nucleic acid-containing sample from the second process chamber after the use of the heater assembly and the magnetic assembly.
2. The apparatus of claim 1 , wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the first process chamber without transporting either the liquid material or the first process chamber to a different location to perform either heater assembly or magnetic assembly operations.
3. The apparatus of claim 2 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane simultaneously.
4. The apparatus of claim 3 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.
5. The apparatus of claim 3 , wherein the magnetic assembly is configured to move relative to the first and second process chambers.
6. The apparatus of claim 3 , wherein the heater assembly is configured to heat the first and second process chambers without unduly heating another portion of the lanes.
7. The apparatus of claim 2 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane in parallel.
8. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a first nucleic acid-containing sample and a second nucleic acid-containing sample, wherein the module comprises:
a first lane comprising a first process chamber;
a waste chamber; and
a second lane comprising a second process chamber;
an assembly, wherein the first process chamber and the second process chamber are configured to be received by the assembly, the assembly comprising:
a magnetic assembly configured to apply a magnetic force to the first process chamber and the second process chamber, and
a heater assembly configured to apply heat to the first process chamber and the second process chamber, wherein the heater assembly is configured to heat the first and second process chambers without unduly heating another portion of the lanes,
wherein, when the first nucleic acid-containing sample and a first plurality of magnetic affinity beads are received in the first process chamber and when the second nucleic acid-containing sample and a second plurality of magnetic affinity beads are received in the second process chamber, the first nucleic acid-containing sample and the first plurality of magnetic affinity beads are configured to be heated within the first process chamber by the heater assembly and wherein the second nucleic acid-containing sample and the second plurality of magnetic affinity beads are configured to be heated within the second process chamber by the heater assembly, and
wherein the magnetic assembly is configured to simultaneously capture or move the first plurality of magnetic affinity beads in the first process chamber and the second plurality of magnetic affinity beads in the second process chamber; and
a liquid dispenser configured to perform suck and dispense operations on the first lane and the second lane.
9. The apparatus of claim 8 , wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the first process chamber without transporting either the liquid material or the first process chamber to a different location to perform either heater assembly or magnetic assembly operations.
10. The apparatus of claim 9 , wherein the magnetic assembly is configured to move relative to the first and second process chambers.
11. The apparatus of claim 10 , wherein the heater assembly is configured to be controlled by a processor.
12. The apparatus of claim 9 , wherein the liquid dispenser is configured to be controlled by a processor.
13. The apparatus of claim 12 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane simultaneously.
14. The apparatus of claim 12 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane in parallel.
15. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a first nucleic acid-containing sample and a second nucleic acid-containing sample, wherein the module comprises:
a first lane comprising a first process chamber;
a waste chamber; and
a second lane comprising a second process chamber;
an assembly, wherein the first process chamber and the second process chamber are configured to be received by the assembly, the assembly comprising:
a magnetic assembly configured to apply a magnetic force to the first process chamber and the second process chamber, and
a heater assembly configured to apply heat to the first process chamber and the second process chamber, wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the first process chamber without transporting either the liquid material or the first process chamber to a different location to perform either heater assembly or magnetic assembly operations,
wherein, when the first nucleic acid-containing sample and a first plurality of magnetic affinity beads are received in the first process chamber and when the second nucleic acid-containing sample and a second plurality of magnetic affinity beads are received in the second process chamber, the first nucleic acid-containing sample and the first plurality of magnetic affinity beads are configured to be heated within the first process chamber by the heater assembly and wherein the second nucleic acid-containing sample and the second plurality of magnetic affinity beads are configured to be heated within the second process chamber by the heater assembly, and
wherein the magnetic assembly is configured to simultaneously capture or move the first plurality of magnetic affinity beads in the first process chamber and the second plurality of magnetic affinity beads in the second process chamber; and
a liquid dispenser configured to perform suck and dispense operations on the first lane and the second lane.
16. The apparatus of claim 15 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane simultaneously.
17. The apparatus of claim 15 , wherein the magnetic assembly is configured to move relative to the first and second process chambers.
18. The apparatus of claim 15 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane in parallel.
19. The apparatus of claim 15 , wherein the liquid dispenser is configured to withdraw an entire solution from each process chamber.
20. The apparatus of claim 15 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.