Polymer compositions for storage and release of polypeptides
The present invention relates to the discovery of novel PLGA particles comprising a polypeptide adsorbed on the surface of the PLGA particles. In certain embodiments, the PLGA particles can be used to administer the polypeptide to a patient in need thereof. As compared to polypeptide-delivery formulations known in the art, the present invention demonstrates a higher relative polypeptide loading capacity. In certain embodiments, the polypeptide adsorbed on the surface of the PLGA nanoparticles does not denature or deform upon adsorption.
1. A method of forming polypeptide-adsorbed poly(lactic-co-glycolic acid) (PLGA) particles,
the method comprising contacting an aqueous suspension comprising PLGA particles with an aqueous solution comprising a polypeptide, whereby the polypeptide is adsorbed on the PLGA particles,
wherein the PLGA particles comprise nanoparticles having a diameter ranging from about 1 nm to about 100 nm;
wherein the PLGA particles comprise a lactic acid-to-glycolic acid ratio from about 1:1 to about 6:1;
wherein the PLGA particles comprise a PLGA polymer having a molecular weight from about 10 kDa to about 50 kDa;
wherein the aqueous solution comprises about 0.001 mg/ml to about 0.2 mg/ml of the polypeptide;
wherein at least a portion of the PLGA particles comprises an ester end-capped PLGA polymer;
wherein the polypeptide comprises a human growth hormone; and
wherein at least a portion of the human growth hormone adsorbed to the surface of the PLGA particles retains its active secondary and tertiary structure as compared to the polypeptide prior to adsorption, and is not significantly denatured by the adsorption process.
2. The method of claim 1 , wherein the human growth hormone comprises a recombinant human growth hormone.
3. The method of claim 1 , wherein the aqueous suspension and the aqueous solution independently have a pH of about 6.5 to about 7.5.
4. The method of claim 1 , wherein the PLGA particles comprise PLGA 5050 or PLGA 8515.
5. The method of claim 1 , wherein at least one of the aqueous suspension and the aqueous solution is buffered.
6. The method of claim 1 , wherein the PLGA particles are selected from the group consisting of PLGA 5050 1A particles, PLGA 5050 5E particles, and PLGA 8515 3CE particles.
7. A composition comprising poly(lactic-co-glycolic acid) (PLGA) particles and a polypeptide;
wherein the polypeptide is adsorbed on at least a fraction of the surface of the PLGA particles;
wherein the PLGA particles comprise nanoparticles having a diameter of about 1 nm to about 100 nm;
wherein the PLGA particles comprise a lactic acid-to-glycolic acid ratio from about 1:1 to about 6:1;
wherein the PLGA particles comprise a PLGA polymer having a molecular weight from about 10 kDa to about 50 kDa;
wherein the PLGA particles comprise an ester end-capped PLGA polymer;
wherein the polypeptide comprises a human growth hormone; and
wherein at least a portion of the human growth hormone adsorbed to the surface of the PLGA particles retains its active secondary and tertiary structure as compared to the polypeptide prior to adsorption, and is not significantly denatured by the adsorption process.
8. The composition of claim 7 , wherein the human growth hormone comprises a recombinant human growth hormone.
9. The composition of claim 7 , wherein the polypeptide adsorbs to the surface of the PLGA particles to form a monolayer or more than one layer.
10. The composition of claim 7 , wherein the polypeptide is not imbedded in the PLGA particles.
11. The composition of claim 7 , wherein the composition comprises a (w/w) ratio of the polypeptide: PLGA of about 1:0.15 to about 1:6.
12. The composition of claim 7 , wherein the PLGA particles are biocompatible or biodegradable.
13. The composition of claim 7 , further comprising at least one pharmaceutically acceptable carrier.
14. The composition of claim 7 , wherein the PLGA particles are selected from the group consisting of PLGA 5050 1A particles, PLGA 5050 5E particles, and PLGA 8515 3CE particles.
15. A method of treating human growth hormone deficiency in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a composition of claim 7 .
16. The method of claim 15 , wherein the human growth hormone is recombinant.
17. A method of delivering a polypeptide to a lung or pair of lungs of a subject in need thereof, the method comprising administering to the lung(s) of the subject a therapeutically effective amount of the composition of claim 7 .