Microarray based sample detection system
A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
1. A microarray assembly for detection of a target molecule in a sample, comprising:
an amplification chamber with a sample inlet, a sample outlet, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface, wherein the microarray is a gel spot microarray, wherein nucleic acids are amplified in the amplification chamber and hybridize to the microarray and wherein the top interior surface is a hydrophilic surface that facilitates complete filling of the amplification chamber by an aqueous fluid;
a one-way valve at the sample inlet to prevent environmental contamination;
a waste chamber comprising a waste inlet and an absorbent material; and
a channel connecting the amplification chamber to the waste chamber, the channel having an expansion section with a first end proximate to the outlet of the amplification chamber and a second end proximate to the inlet of the waste chamber,
wherein the top interior surface of the amplification chamber is a hydrophilic surface that facilitates complete filling of the amplification chamber by an aqueous fluid and wherein the cross-sectional area at the first end of the expansion section is smaller than the cross-sectional area at the second end of the expansion section.
2. The microarray assembly of claim 1 , wherein the expansion section of the channel has cross-sectional areas that increase stepwisely from the first end to the second end.
3. The microarray assembly of claim 1 , wherein the channel further comprises a switchback section between the expansion section and the inlet of the waste chamber.
4. The microarray assembly of claim 3 , wherein the switchback section comprises two sharp turns in the channel.
5. The microarray assembly of claim 1 , wherein the one-way valve is a dome valve.
6. The microarray assembly of claim 1 , wherein microarray assembly comprises a substrate layer, a cover layer and a spacer layer located between the substrate layer and the cover layer.
7. The microarray assembly of claim 6 , wherein the spacer layer is a doublesided tape and wherein the amplification chamber is formed within the spacer layer.
8. The microarray assembly of claim 1 , wherein the microarray assembly comprises a substrate layer and a cover layer, wherein the substrate layer is an injection molded plastic with features that create walls of the amplification chamber and a pocket for the waste chamber.
9. The microarray assembly of claim 8 , wherein the cover, layer is a hydrophilic film laminated to the substrate layer.
10. The microarray assembly of claim 1 , wherein the gel spot array comprises a plurality of gel spots and wherein each gel spot comprises an internal control fluorophore and a capturing agent that binds specifically to a target molecule.
11. The microarray, assembly of claim 1 , wherein the gel spot microarray is a nucleotide array.