Cell processing unit, cell processing system and methods of use thereof
The disclosure relates to a cell processing unit for cell and gene therapy manufacture comprising a housing defining an enclosure into which a cell processing platform can be mounted, a platform mounting bracket within the housing and configured and arranged to receive and retain a cell processing platform, a drive apparatus configured and arranged to operatively engage and act upon a cell processing platform to move same with respect to the platform mounting bracket, and an actuator configured and arranged to exert a force on a container mounted into the cell processing platform to expel a contents from the container.
1. A cell processing unit for cell and gene therapy manufacture, comprising;
a housing defining an enclosure into which a cell processing platform is mountable;
a platform mounting bracket within the housing and configured and arranged to receive and retain the cell processing platform with one or more auxiliary containers and a primary container mounted to the cell processing platform;
a primary actuator configured and arranged to exert a force on the primary container mounted to the cell processing platform;
a rotational drive apparatus configured and arranged to operatively engage and act upon the cell processing platform to rotate the cell processing platform with respect to the platform mounting bracket; and
an auxiliary actuator configured and arranged to exert a force on an auxiliary container mounted to the cell processing platform to expel a contents from the auxiliary container.
2. The cell processing unit of claim 1 , wherein the platform mounting bracket comprises a mounting plate.
3. The cell processing unit of claim 2 , wherein the platform mounting bracket comprises a mounting flange spaced apart from the mounting plate to receive and retain the cell processing platform in a position in the housing between the mounting plate and the mounting flange.
4. The cell processing unit of claim 2 , wherein the mounting plate is substantially C-shaped.
5. The cell processing unit of claim 1 , wherein the rotational drive apparatus comprises a drive wheel that is mounted on the platform mounting bracket and is configured to engage a surface of the cell processing platform and to impart rotational movement on the cell processing platform.
6. The cell processing unit of claim 5 , wherein the rotational drive apparatus comprises a sprung wheel biased toward the drive wheel and spaced apart from the drive wheel and mounted on the platform mounting bracket.
7. The cell processing unit of claim 5 , wherein the rotational drive apparatus comprises a hinged wheel biased toward the drive wheel and spaced apart from the drive wheel and mounted on the platform mounting bracket.
8. The cell processing unit of claim 7 , wherein the hinged wheel is moveable into an open position in which the cell processing platform is insertable and engageable with the cell processing platform mounting bracket, and wherein the hinged wheel is moveable into a closed position in which the hinged wheel is engaged with the surface of the cell processing platform to retain the cell processing platform in the cell processing platform mounting bracket.
9. The cell processing unit of claim 1 , wherein at least one of the auxiliary actuator and the primary actuator is a linear actuator.
10. The cell processing unit of claim 9 , wherein the linear actuator comprises a plunger operatively coupled to a drive motor, wherein the plunger is configured to engage the auxiliary container mounted to the cell processing platform and to exert a compression force on the auxiliary container.
11. The cell processing unit of claim 1 , wherein the primary actuator is configured to expel a fluid from the primary container.
12. The cell processing unit of claim 1 , wherein:
the cell processing platform includes a base plate, the auxiliary container, and the primary container, the auxiliary container and the primary container being positioned at opposite ends of the base plate;
the platform mounting bracket includes an opening formed therein, the opening extending from a side thereof for receiving the base plate;
the primary actuator is positioned below the opening formed in platform mounting bracket; and
the auxiliary actuator is positioned above the opening of the platform mounting bracket.
13. The cell processing unit of claim 12 , wherein the primary actuator comprises a plunger operatively coupled to a drive motor, wherein the plunger is configured to engage the primary container mounted to the cell processing platform and to exert a compression force on the primary container.
14. The cell processing unit of claim 1 , comprising a location detecting sensor operable to detect a position of the cell processing platform relative to the platform mounting bracket.
15. The cell processing unit of claim 1 , comprising a location detecting sensor operable to detect a rotational position of the cell processing platform relative to the platform mounting bracket.
16. The cell processing unit of claim 1 , wherein one or more of the primary container and the one or more auxiliary containers mounted to the cell processing platform is compressible.
17. The cell processing unit of claim 1 , wherein one or more of the primary container and the one or more auxiliary containers mounted to the cell processing platform respectively comprises: a base section, a top section arranged substantially in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, wherein the wall element is compressible with respect to the top and base section and the wall element comprises a flexible material.
18. The cell processing unit of claim 1 , the container mounted to the cell processing unit is one of: a reagent container, a bioreactor, a cell culture container, a waste container, a filter, an electroporator, a purifier, holding container, apheresis/leukopheresis, differentiation chamber, chromatography column, settling chamber, sieve, shaking/mixer, a centrifuge or a magnetic bead separator.
19. The cell processing unit of claim 1 , wherein control of the cell processing unit is automated.
20. A cell processing unit for cell and gene therapy manufacture, comprising;
a housing defining an enclosure into which a cell processing platform is mountable;
a platform mounting bracket within the housing and configured and arranged to receive and retain the cell processing platform;
a rotational drive apparatus configured and arranged to operatively engage and act upon the cell processing platform to rotate the cell processing platform with respect to the platform mounting bracket, the rotational drive apparatus comprising:
a drive wheel that is mounted on the platform mounting bracket and is configured to engage a surface of the cell processing platform and to impart rotational movement on the cell processing platform; and
a hinged wheel biased toward the drive wheel and spaced apart from the drive wheel and mounted on the platform mounting bracket; and
an actuator configured and arranged to exert a force on a container mounted to the cell processing platform to expel a contents from the container.