Automatic system and method for tissue sectioning, staining, and scanning
Disclosed is plate or film for collecting and analyzing a sample. The plate or film includes a surface and a plurality of slices from the sample immobilized on the surface. Further, the plurality of slices are cut from the sample with equal thickness, and the plurality of slices are placed on the surface of the plate or film following their cutting order.
1. A method for processing a biological sample, comprising:
transferring a plurality of slices cut from the biological sample to a collecting film, the plurality of slices being transferred sequentially onto the collecting film;
spooling, at least while the plurality of slices are being transferred to the collecting film, the collecting film to form a rolled collecting film, the rolled collecting film having a plurality of rolled layers, each rolled layer of the plurality of rolled layers comprising a portion of the collecting film and a portion of the plurality of slices;
staining the plurality of slices collectively while the plurality of slices are positioned on the rolled collecting film;
unwinding the rolled collecting film; and
imaging the plurality of slices on the collecting film.
2. The method of claim 1 , wherein the biological sample is a formalin-fixed, paraffin-embedded (FFPE) tissue.
3. The method of claim 2 , further comprising deparaffinizing the plurality of slices.
4. The method of claim 3 , wherein deparaffinizing the plurality of slices comprises deparaffinizing the plurality of slices collectively while the plurality of slices are positioned on the rolled collecting film.
5. The method of claim 1 , wherein the imaging further comprises imaging each slice of the plurality of slices at a speed of between 10 slices per minute to 60 slices per minute.
6. The method of claim 1 , wherein the plurality of slices are juxtaposed on a surface of the collecting film that follows a cutting order of the plurality of slices.
7. The method of claim 1 , wherein staining the plurality of slices comprises submerging the rolled collecting film in a reagent bath.
8. The method of claim 7 , wherein spooling the collecting film comprises winding the collected film onto a staining spiral to form to the rolled collecting film, and wherein the staining spiral is submerged with the rolled collecting film in a reagent bath.
9. The method of claim 1 , further comprising, rolling the collecting film with the plurality of slices onto a storage spool.
10. The method of claim 9 , further comprising, subsequent to unwinding the rolled collecting film and before rolling the collecting film with the plurality of slices onto a storage spool, applying a sealant to the plurality of slices.
11. The method of claim 9 , further comprising applying a cover to the plurality of slices on the collecting film before rolling the collecting film with the plurality of slices onto a storage spool.
12. The method of claim 1 , wherein unwinding the rolled collecting film includes positioning the collecting film for a plurality of equidistantly spaced holes along a pair of opposing lateral edges of the collecting film to sequentially receive a portion of a sprocket as the collecting film is displaced to at least a location at which the imaging is conducted.
13. The method of claim 1 , further comprising cutting the biological sample into a plurality of slices with equal thickness.
14. The method of claim 1 , further comprising amplifying an analog image of each slice of the plurality of slices prior to imaging of the slice, the analog image comprising a high-resolution, polychromatic array.
15. The method of claim 1 , further comprising
rerolling, following imaging of the plurality of slices, the collecting film containing the plurality of slices onto a spool.
16. The method of claim 1 , wherein imaging the plurality of slices on the collecting film occurs during the unwinding of the rolled collecting film.
17. The method of claim 1 , wherein the imaging is conducted individually for each slice of the plurality of slices at a time when moving the collecting film through the imaging device.
18. The method of claim 1 , wherein for each slice of the plurality of slices the imaging device captures a single image comprising an entirety of the slice.
19. The method of claim 1 , wherein spooling further comprises separating each rolled layer of the plurality of rolled layers from an adjacent rolled layer by a space.