Granted Patent
B2
US 12,692,480 · App. 17/449,762 · Granted Jul 28, 2026
Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
Inventors:
Martin Yarmush (Newton, MA); Basak E. Uygun (Cambridge, MA); Korkut Uygun (Boston, MA); Maria-Louisa Izamis (Boston, MA); Alejandro Soto-Gutierrez (Boston, MA); Herman Tolboom (Zurich, CH); Hiroshi Yagi (Brookline, MA); Carley Shulman (Sudbury, MA); Jack Milwid (Denver, CO)
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Abstract
One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.
Claims (16)
1 . A method for harvesting adult cells from an organ, the method comprising:
(a) perfusing the organ with a perfusate comprising an enzymatic digestion agent and one or more anti-inflammatory agents, wherein the one or more anti-inflammatory agents comprise hydrocortisone;
(b) isolating the adult cells from the organ; and
(c) culturing the adult cells, thereby harvesting the adult cells from the organ.
2 . The method of claim 1 , wherein the organ is a mammalian organ.
3 . The method of claim 2 , wherein the organ is a human organ.
4 . The method of claim 1 , wherein the organ is one selected from the group consisting of: spleen, heart, lung, and kidney.
5 . The method of claim 1 , wherein the organ is ischemic and/or wherein the organ is a damaged organ.
6 . The method of claim 1 , wherein the perfusate includes one or more oxygen carriers, wherein the one or more oxygen carriers comprise erythrocytes.
7 . The method of claim 1 , wherein the enzymatic digestion agent comprises collagenase.
8 . The method of claim 7 , wherein the perfusate is a normothermic perfusate, a room temperature perfusate, or a hypothermic perfusate.
9 . The method of claim 7 , further comprising providing the organ.
10 . The method of claim 7 , further comprising filtering the perfusate through one or more mesh filters having pore sizes ranging from about 1 μm to about 1000 μm.
11 . The method of claim 7 , further comprising performing density centrifugation on the perfusate, or wherein the method further comprises circulating the perfusate through a dialyzer.
12 . The method of claim 7 , wherein the perfusing step improves vitality of the adult cells.
13 . The method of claim 1 , wherein the enzymatic digestion agent is selected from the group consisting of collagenase I, collagenase II, collagenase III, collagenase IV, collagenase V, collagenase VI, trypsin, and hyaluronidase.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 29, 2026
From: SHULMAN, CARLEY
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 074800/0626 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 20, 2021
From: IZAMIS, MARIA LOUISA; MILWID, JACK
To: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Reel/Frame 057846/0046 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 20, 2021
From: YARMUSH, MARTIN; UYGUN, BASAK E.; UYGUN, MUSFATA KORKUT; SOTO-GUTIERREZ, ALEJANDRO; TOLBOOM, HERMAN; YAGI, HIROSHI
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 057846/0146 →
Continuity (6)
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