Granted Patent B2

US 12,195,392 · App. 17/570,312 · Granted Jan 14, 2025

Inventors: Ginger K. Dosier (Raleigh, NC); Victoria M. Durham (San Diego, CA); Thomas A. Hill (Dacula, GA); J. Michael Dosier (Raleigh, NC); Steven W. McAllister (Durham, NC)

Assignee: Biomason Inc.

C04B12/00B05D1/02C04B28/10C12N11/02C12N11/14C12Y305/01005C04B2103/0001C04B2103/0075C04B2111/00146C04B2111/60Y02W30/91

Abstract

Claims (26)

1. A method of dust control, comprising:

determining a temperature of use;

selecting an amount of nitrogen and calcium sources and an amount of enzyme to solidify a slurry within a particular time frame based on the temperature of use, the nitrogen source comprises urea;

creating a composition that includes the amount of nitrogen and calcium sources and spores for producing the amount of enzyme, the spores comprise spores of a urease-producing microorganism;

distributing the composition over a surface, the distributing the composition includes distributing the nitrogen and calcium sources in a timed-release form with a pre-determined rate of dissolution;

germinating the spores on the surface to produce calcite to form a self-renewable crust at the surface; and

generating the slurry to recreate the self-renewable crust at the surface by applying water to the surface without the reapplication of the composition to the surface.

2. The method of claim 1 , wherein the composition includes nutrients that promote the proliferation of the spores.

3. The method of claim 2 , wherein the distributing the composition includes distributing the nutrients in a timed-release form with a particular rate of dissolution.

4. The method of claim 1 , wherein the composition includes a transport medium which maintains viability of the spores without propagation and support material that is inorganic and porous.

5. The method of claim 1 , wherein the composition includes aggregate of only 0.5 mm or less in diameter.

6. The method of claim 1 , wherein the surface is more resistant to erosion after applying the composition.

7. The method of claim 1 , wherein the surface is a walking path, a pile, a cliff or vehicle roadway.

8. The method of claim 1 , wherein the composition is a liquid, a gel, a slurry, a sludge, a semisolid, or dry powder.

9. The method of claim 1 , wherein the spores are spores of Sporosarcina pasteurii, Sporosarcina ureae, Proteus vulgaris, Bacillus sphaericus, Myxococcus xanthus, Proteus mirabilis, Helicobacter pylori , or variants, serotypes, mutations or combinations thereof.

10. The method of claim 2 , wherein the nutrients comprise one or more of water, vitamins, minerals, amino acids, proteins, oils, fatty acids, saccharides and polysaccharides.

11. The method of claim 1 , wherein the distributing comprises spraying, coating, misting, or painting the composition onto the surface or immersing the surface into the composition.

12. The method of claim 1 , wherein the calcium source comprises calcium chloride, calcium acetate, calcium phosphate, calcium carbonate, calcium lactate, or calcium nitrate.

13. The method of claim 2 , wherein the spores are encapsulated or coated with the nutrients.

14. The method of claim 1 , wherein the surface comprises sand, gravel, rock, dirt, glass, wood, paper, metal, plastic, polymers, fibers, minerals or combinations thereof.

15. The method of claim 1 , wherein the surface is a natural geological or man-made surface comprising a cliff, a dune, an aggregate pile, a ledge, a supporting wall, a foundation, tailings, piles of waste materials from a manufacturing process, or a structure for which support or reinforcement is desired.

16. The method of claim 1 , wherein the composition comprises a coloring agent, an identifying agent or a detectable marker.

17. The method of claim 1 , wherein the distributing the composition to the surface reduces dust and other particulate materials generated from the surface as compared to an unapplied surface.

18. The method of claim 17 , wherein the dust and other particulate materials generated from the surface comprise sand, glass, wood, pulp, sawdust, lignin, metal, polymers, fines, micro-cellulose, waste materials, ash, scrubber waste, biomass, moss, hay, straw, grass, sticks, leaves, algae, dirt, ash, particulate material, refuse, spores, carbon, soot, materials from cement, block, or brick manufacturing, materials from foundry operations, materials from grinding limestone, materials from sand tailings, materials from mining, materials from smelters, materials from paint manufacturing, byproducts from manufacturing operations, or combinations thereof.

19. The method of claim 17 , wherein calcium carbonate formed at the surface covers or encapsulates the dust.

20. The method of claim 1 , wherein the applying the composition reduces or eliminates dust.

Assignments (1)

Continuity (6)

References Cited (263)