Free polyunsaturated fatty acid-containing composition and manufacturing method therefor
The present disclosure is: a free polyunsaturated fatty acid-containing composition, which comprises at least one free polyunsaturated fatty acid having 20 or more carbon atoms, the content being at least 80.0% of the fatty acids in the composition, and satisfies at least one selected from a group consisting of conditions (1) and (2): (1) the content of conjugated unsaturated fatty acid is 1.0% or less of the fatty acids in the composition, and (2) the Gardner color is less than 3+; and a manufacturing method for the free polyunsaturated fatty acid-containing composition comprising the preparation of a raw material composition containing at least one polyunsaturated fatty acid having 20 or more carbon atoms, and hydrolysis of a reaction solution containing the prepared raw material composition, a lower alcohol, water and an alkali catalyst at a temperature of 10° C. or lower.
1. A manufacturing method of a composition comprising free eicosapentaenoic acid, the method comprising:
providing a raw material composition containing eicosapentaenoic acid; and
performing hydrolysis treatment on a reaction solution containing the provided raw material composition, a lower alcohol, water, and an alkali catalyst in a temperature condition at 10° C. or lower, to obtain the composition comprising free eicosapentaenoic acid.
2. The manufacturing method according to claim 1 , wherein the raw material composition comprises eicosapentaenoic acid in a form of an eicosapentaenoic alkyl ester.
3. The manufacturing method according to claim 1 , the method further comprising adding an acid in a reaction solution after the hydrolysis treatment to terminate the hydrolysis reaction, a pH of the reaction solution after the acid addition being from pH 1.0 to 6.0.
4. The manufacturing method according to claim 1 , wherein an amount of the lower alcohol in the reaction solution is from 0.9 equivalents to 32.0 equivalents relative to an amount of total fatty acids in the raw material composition.
5. The manufacturing method according to claim 1 , wherein an amount of the lower alcohol in the reaction solution is from 0.20 to 8.20 in terms of weight content ratio relative to the water.
6. The manufacturing method according to claim 1 , wherein an amount of the water in the reaction solution is from 6.0 equivalents to 13.0 equivalents relative to an amount of total fatty acids in the raw material composition.
7. The manufacturing method according to claim 1 , wherein an amount of the alkali catalyst in the reaction solution is from 1.0 equivalent to 2.3 equivalents relative to an amount of total fatty acids in the raw material composition.
8. The manufacturing method according to claim 1 , wherein the alkali catalyst is at least one selected from the group consisting of sodium hydroxide and potassium hydroxide.
9. The manufacturing method according to claim 1 , wherein the temperature condition of the hydrolysis treatment is from −20° C. to 10° C.
10. The manufacturing method according to claim 1 , wherein the raw material composition is derived from a microbial raw material.