IP Library Granted Patent US 12,331,079
Granted Patent B2
US 12,331,079 · App. 17/893,052 · Granted Jun 17, 2025

Oncolytic virus platform to treat cancers with myxoma virus

Inventors: Douglas Grant McFadden (Tempe, AZ); Mohammed Masmudur Rahman (Chandler, AZ); Nancy Villa (Tempe, AZ); Lino Torres-Dominguez (Tempe, AZ); Lina Franco Achury (Tempe, AZ); Leslie Lynne Sharp (Rancho Santa Fe, CA)
Assignee: Arizona Board of Regents on behalf of Arizona State University
C07K14/005A61K39/275A61K45/06A61P35/04C07K14/525C07K14/5434C12N7/00A61K2039/5154A61K2039/5256A61K2039/545A61K2039/572A61K2039/585C07K2319/33C12N2710/24022C12N2710/24033C12N2710/24071
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Quick Facts
Patent No.
US 12,331,079
App. No.
17/893,052
Granted
Jun 17, 2025
Kind
B2
Abstract

Disclosed herein, in certain embodiments, are recombinant myxoma viruses (MYXVs) and nucleic acid constructs encoding the recombinant MYXVs. In some embodiments, the MYXVs are engineered to inactivate or attenuate an activity or expression level of an M153 protein. In some embodiments, the MYXVs are engineered to express one or more transgenes such as a tumor necrosis factor (TNF), interleukin-12 (IL-12), or decorin. Also disclosed herein, in certain embodiments, are methods of using the MYXVs. Some embodiments include providing a MYXV as described herein to a subject in need thereof.

Claims (22)

1. A pharmaceutical composition comprising:

i) a myxoma virus (MYXV) comprising a genetic modification at its M153 locus and a transgene encoding a cytokine or a cell matrix protein; and

ii) a pharmaceutically acceptable excipient.

2. The pharmaceutical composition of claim 1 , wherein the genetic modification comprises a disruption of M153 gene.

3. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated for systemic administration.

4. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated for local administration.

5. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated for parenteral administration.

6. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises the MYXV at a dose of less than 1×10 9 focus forming units (FFU).

7. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises the MYXV at a dose of at least 1×10 7 focus forming units (FFU).

8. The pharmaceutical composition of claim 1 , wherein upon administration of the MYXV to a subject, the transgene is effective to increase expression of at least two cytokines in a cancer cell of the subject at least about 30% compared to before the administering.

9. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises the MYXV at a dose effective to increase expression of at least two cytokines in a cancer cell of a subject at least about 30% upon administration of the MYXV to the subject.

10. The pharmaceutical composition of claim 8 , wherein the at least two cytokines comprise IFN-γ, IL-2, IL-6, IL-10, IL-12, or TNF-α.

11. The pharmaceutical composition of claim 1 , wherein the MYXV is effective to reduce viability of a cancer cell.

12. The pharmaceutical composition of claim 8 , wherein the cancer cell comprises a solid tumor cell.

13. The pharmaceutical composition of claim 8 , wherein the cancer cell comprises an osteosarcoma, triple negative breast cancer, or melanoma cell.

14. The pharmaceutical composition of claim 1 , wherein the transgene encodes the cytokine.

15. The pharmaceutical composition of claim 8 , wherein the at least two cytokines comprise TNF-α and IL-12.

16. The pharmaceutical composition of claim 1 , wherein the transgene encodes human TNFα.

17. The pharmaceutical composition of claim 1 , wherein the transgene encodes human IL-12.

18. The pharmaceutical composition of claim 1 , wherein the transgene encodes the cell matrix protein.

19. The pharmaceutical composition of claim 1 , wherein the transgene encodes human decorin.

20. The pharmaceutical composition of claim 1 , wherein the transgene encodes the cytokine and the MYXV further comprises a second transgene that encodes the cell matrix protein.

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 18, 2024
From: ONCOMYX THERAPEUTICS, INC.
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 069626/0388 →
CONFIRMATORY LICENSE Recorded Jul 25, 2024
From: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
To: NATIONAL INSTITUTES OF HEALTH
Reel/Frame 068169/0433 →
CORRECTIVE ASSIGNMENT TO CORRECT THE RECEIVING PARTY DATA PREVIOUSLY RECORDED AT REEL: 64592 FRAME: 965. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Feb 21, 2024
From: SHARP, LESLIE LYNNE
To: ONCOMYX THERAPEUTICS, INC.
Reel/Frame 066644/0555 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 15, 2023
From: MCFADDEN, DOUGLAS GRANT; RAHMAN, MOHAMMED MASMUDUR; VILLA, NANCY; TORRES-DOMINGUEZ, LINO; FRANCO ACHURY, LINO
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 064591/0981 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 15, 2023
From: SHARP, LESLIE LYNNE
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 064592/0965 →
Continuity (5)
Continuation 17357624 · Jun 24, 2021
Continuation 17010711 · Sep 2, 2020
Provisional Application 62944233 · Dec 5, 2019
Provisional Application 62894925 · Sep 2, 2019
Related Publication 20230025706A1 · Jan 26, 2023
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