IP Library › Granted Patent US 12,291,741
Granted Patent B2
US 12,291,741 · App. 17/973,055 · Granted May 6, 2025

Detection units and methods for detecting a target analyte

Inventors: Alfredo Andres Celedon (Columbia, MD); Saravana Radha Krishna Murthy (Gaithersburg, MD); Zhiguang Xu (Baltimore, MD); Danielle Elise Schultz (Gaithersburg, MD); Troy Allen Horn (Reisterstown, MD)
Assignee: Scanogen Inc.
C12Q1/6816G01N27/3271G01N27/3276G01N27/4145G01N27/745G01N33/54306G01N33/54313G01N33/54326G01N33/54373
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Quick Facts
Patent No.
US 12,291,741
App. No.
17/973,055
Granted
May 6, 2025
Kind
B2
Abstract

The present application relates to detection units and methods for detecting one or more target analytes in a sample using a complex formed by a target and first and second probes, wherein the complex comprises an elongated region, a particle that is coupled to the first probe, and a solid support that is coupled to the second probe. Specific binding of a target analyte can be distinguished from non-specific binding of the particle by measuring the displacement of the particle.

Claims (29)

1. A kit for detecting a target analyte in a sample, comprising:

a) a first probe comprising a first region configured to directly bind the target analyte at a first location and a second region configured to couple to a particle, wherein the first probe cannot specifically couple to a solid support in the absence of the target analyte; and

b) a second probe comprising a first region configured to directly bind the target analyte at a second location different than the first location, and a second region configured to couple to the solid support, wherein the second probe cannot specifically couple to the particle in the absence of the target analyte;

wherein the first probe and the second probe can bind to the target analyte, if present in the sample, to form a complex such that the particle can be indirectly coupled to the solid support, and wherein the length of the complex from where the first probe couples to the particle to where the second probe couples to the solid support is about 1.0 micron to about 20 microns.

2. The kit of claim 1 , wherein the first probe comprises a first antibody and the second probe comprises a second antibody.

3. The kit of claim 1 , wherein the first probe comprises a first nucleic acid and the second probe comprises a second nucleic acid.

4. The kit of claim 1 , wherein the target analyte is a protein.

5. The kit of claim 1 , wherein the target analyte is a nucleic acid molecule.

6. A kit for detecting a target analyte in a sample, comprising:

a) a first probe comprising a first region configured to directly bind the target analyte at a first location and a second region configured to couple to a detectable particle, wherein the first probe cannot specifically couple to a solid support in the absence of the target analyte; and

b) a second probe comprising a first region configured to directly bind the target analyte at a second location different than the first location, and a second region configured to couple to the solid support, wherein the second probe cannot specifically couple to the detectable particle in the absence of the target analyte;

wherein the first probe and the second probe can bind to the first and second locations on the target analyte, if present in the sample, to form a complex of the first probe, the second probe, the target analyte, and the detectable particle, and wherein the complex can couple to the solid support; and wherein the length of the complex from where the first probe couples to the detectable particle to where the second probe couples to the solid support is about 1.0 micron to about 20 microns.

7. The kit of claim 6 , wherein the first probe comprises a first antibody and the second probe comprises a second antibody.

8. The kit of claim 6 , wherein the first probe comprises a first nucleic acid and the second probe comprises a second nucleic acid.

9. The kit of claim 6 , wherein the target analyte is a protein.

10. The kit of claim 6 , wherein the target analyte is a nucleic acid molecule.

11. A method of detecting a target analyte in a sample comprising:

a) providing a detectable particle indirectly coupled to a solid support via a complex if the target analyte is present in the sample, the complex formed from:

i) a first probe comprising a first region configured to directly bind the target analyte at a first location and a second region configured to couple to the detectable particle, wherein the first probe cannot specifically couple to the solid support in the absence of the target analyte; and

ii) a second probe comprising a first region configured to directly bind the target analyte at a second location different than the first location, and a second region configured to couple to the solid support, wherein the second probe cannot specifically couple to the detectable particle in the absence of the target analyte;

iii) the target analyte,

wherein at least one of the first probe, the second probe, and a region of the target analyte between the first location and the second location comprises an elongated region of about 1.0 micron to about 20 microns long;

b) either i) applying a force to the complex or ii) measuring the Brownian motion of the detectable particle.

12. The method of claim 11 , wherein the first probe comprises a first antibody and the second probe comprises a second antibody.

13. The method of claim 11 , wherein the first probe comprises a first nucleic acid and the second probe comprises a second nucleic acid.

14. The method of claim 11 , wherein the first probe comprises the elongated region.

15. The method of claim 11 , wherein the second probe comprises the elongated region.

16. The method of claim 11 , wherein the region of the target analyte between the first location and the second location comprises the elongated region.

17. The method of claim 11 , wherein the target analyte is a protein or a nucleic acid molecule.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 21, 2022
From: CELEDON, ALFREDO ANDRES; KRISHNA MURTHY, SARAVANA RADHA; XU, ZHIGUANG; SCHULTZ, DANIELLE ELISE; HORN, TROY ALLEN
To: SCANOGEN INC.
Reel/Frame 062167/0839 →
Continuity (4)
Continuation 16217253 · Dec 12, 2018
Continuation 15033629
Provisional Application 61936863 · Feb 6, 2014
Related Publication 20230039492A1 · Feb 9, 2023
References Cited (70)
US 5525468A · McSwiggen · 1996 [cited by applicant]
US 7736889B2 · Rife · 2010 [cited by applicant]
US 9040287B2 · Chang et al. · 2015 [cited by applicant]
US 9382578B2 · Celedon · 2016 [cited by applicant]
US 9382580B2 · Celedon · 2016 [cited by applicant]
US 10179930B2 · Celedon · 2019 [cited by examiner]
US 10768406B2 · Xu et al. · 2020 [cited by applicant]
US 11371986B2 · Celedon · 2022 [cited by examiner]
US 11505818B2 · Celedon · 2022 [cited by examiner]
US 20020115088A1 · Kum · 2002 [cited by applicant]
US 20030207296A1 · Park · 2003 [cited by examiner]
US 20040073120A1 · Motz et al. · 2004 [cited by applicant]
US 20040234970A1 · Yoo · 2004 [cited by applicant]
US 20100234234A1 · Ferrigno et al. · 2010 [cited by applicant]
US 20100253328A1 · Celedon et al. · 2010 [cited by applicant]
US 20100267169A1 · Hajimiri · 2010 [cited by applicant]
US 20110015380A1 · Vezenov · 2011 [cited by applicant]
US 20120115744A1 · Raymond · 2012 [cited by applicant]
US 20120248292A1 · Ozcan et al. · 2012 [cited by applicant]
US 20120312957A1 · Loney et al. · 2012 [cited by applicant]
US 20130294645A1 · Sibarita · 2013 [cited by applicant]
US 20140030737A1 · Holmes et al. · 2014 [cited by applicant]
US 20140327687A1 · Murakami · 2014 [cited by applicant]
US 20140354994A1 · Ahner et al. · 2014 [cited by applicant]
US 20150275215A1 · Cerchia et al. · 2015 [cited by applicant]
US 20160076083A1 · Ellington et al. · 2016 [cited by applicant]
US 20160258003A1 · Celedon et al. · 2016 [cited by applicant]
US 20160304943A1 · Isgut · 2016 [cited by applicant]
US 20160350937A1 · Balagurusamy et al. · 2016 [cited by applicant]
US 20170362645A1 · Prins et al. · 2017 [cited by applicant]
US 20170363851A1 · Xu et al. · 2017 [cited by applicant]
US 20180246089A1 · Chou et al. · 2018 [cited by applicant]
US 20190112641A1 · Celedon et al. · 2019 [cited by applicant]
US 20190361016A1 · Celedon et al. · 2019 [cited by applicant]
US 20200400932A1 · Xu et al. · 2020 [cited by applicant]
US 20220291212A1 · Celedon et al. · 2022 [cited by applicant]
EP 1593745A1 · 2005 [cited by applicant]
WO WO1999036577A1 · 1999 [cited by applicant]
WO WO2000046357A1 · 2000 [cited by applicant]
WO WO2000061803A1 · 2000 [cited by applicant]
WO WO2002059364A1 · 2002 [cited by applicant]
WO WO2010021639A1 · 2010 [cited by applicant]
WO WO2011045570A2 · 2011 [cited by examiner]
WO WO2011087916A2 · 2011 [cited by applicant]
WO WO2012071428A2 · 2012 [cited by applicant]
WO WO2013059044A1 · 2013 [cited by examiner]
WO WO2015120147A1 · 2015 [cited by applicant]
WO WO2015195404A1 · 2015 [cited by applicant]
WO WO2019226841A1 · 2019 [cited by applicant]
Ahern, “Biochemical, Reagents Kits Offer Scientists Good Return On Investment”, The Scientist, vol. 9, No. 15), 7 pages (1995). [cited by applicant]
Beveridge et al., “The Use of Magnetic Nanoparticles in Analytical Chemistry”, Annu. Rev. Anal. Chem., vol. 4, pp. 251-273 (2011). [cited by applicant]
Celedon et al., “Magnetic tweezers measurement of single molecule torque”, Nano. Lett., vol. 9, No. 4, pp. 1720-1725 (2009). [cited by applicant]
Celedon et al., “Torsional mechanics of DNA are regulated by small-molecule intercalation”, J. Phys. Chem. B, vol. 114, No. 50, pp. 16929-16935 (2010). [cited by applicant]
Coelho et al., “Nuclear segmentation in microscope cell images: A hand-segmented dataset and comparison of algorithms”, Proc. IEEE Int. Symp. Biomed. Imaging, Author Manuscript, doi:10.1109/ISBI.2009.5193098, 8 pages (2… [cited by applicant]
“DNA, chromatin and genome Numbers”, Data taken from leading textbooks and Belmont and Bruce, [cited by applicant]
Dietrich et al., “A New Optical Method For Characterizing Single Molecule Interactions Based On Dark Field Microscopy”, Proc. SPIE, vol. 6444, Ultrasensetive and Single Molecule Detection Technologies II, No. 644403, do… [cited by applicant]
Gusphyl A. Justin et al., “Hydrodynamic focusing for impedance-based detection of specifically bound microparticles and cells: Implications of fluid dynamics on tunable sensitivity”, Sensors and Actuators B: Chemical, v… [cited by applicant]
Han et al., “Elongated oligonucleotide-linked immunosorbent assay for sensitive detection of a biomarker in a microwell plate-based platform”, Biosens. Bioelectron., vol. 50, No. 421-424 (2013). [cited by applicant]
International Search Report and Written Opinion from International Application No. PCT/US2012/059497 mailed May 11, 2015, 11 pages, Application now published as International Publication No. WO2015/120147 on Aug. 13, 20… [cited by applicant]
International Search Report from International Application No. PCT/US2019/033645 mailed Aug. 15, 2019, application now published as International Publication No. WO2019/226841 published as Nov. 28, 2019. [cited by applicant]
Lipfert et al., “Quantitative modeling and optimization of magnetic tweezers”, Biophys. J., vol. 96, No. 12, pp. 5040-5049 (2009). [cited by applicant]
Lipfert et al., “Torsional sensing of small-molecule binding using magnetic tweezers”, Nucl. Acids Res., vol. 38, No. 20, pp. 7122-7132 (2010). [cited by applicant]
Mulvaney et al., “Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics”, Biosens. Bioelectron., vol. 23, No. 2, pp. 191-200 (2007). [cited by applicant]
Mulvaney et al., “Direct Detection Of Genomic DNA With DNA With Fluidic Force Discrimination Assays”, Anal. Biochem., vol. 392, No. 2, pp. 139-144 (2009). [cited by applicant]
Pulido et al., “Magnetic Tweezers”, Encyclopedia of Life Sciences (2011). [cited by applicant]
Revyakin et al., “Single-molecule DNA nanomanipulation: improved resolution through use of shorter DNA fragments”, Nat. Methods, vol. 2, No. 2, pp. 127-138 (2005). [cited by applicant]
Silver et al., “Tethered-bead, immune sandwich assay”, Biosens. Bioelectron., vol. 63, pp. 117-123 (2015). [cited by applicant]
Smith et al., “Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads”, Science, vol. 258, No. 5085, pp. 1122-1126 (1992). [cited by applicant]
Supplementary European Search Report dated May 11, 2017, in European Application No. EP17746242. [cited by applicant]
Yang et al., “Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification”, Biosens. Bioelectron., vol. 27, No. 1, pp. 119-124 (2011). [cited by applicant]