Methods for modulating an intensity profile of a laser beam and systems for same
Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.
1. A system comprising;
a laser;
an acousto-optic device;
a waveform generator configured to input a waveform into the acousto-optic device to generate an output laser beam comprising a plurality of angularly deflected laser beams;
a photodetector; and
a controller comprising a processor having memory operably coupled to the processor wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to:
determine an intensity profile of the output laser beam along a horizontal axis with the photodetector; and
adjust one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile.
2. The system according to claim 1 , wherein the photodetector is an imaging sensor.
3. The system according to claim 1 , wherein the imaging sensor is a camera.
4. The system according to claim 1 , the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to generate an intensity profile plot of the output laser beam.
5. The system according to claim 2 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to generate an intensity profile plot from a captured image of the output laser beam.
6. The system according to claim 1 , wherein the acousto-optic device is an acousto-optic deflector.
7. The system according to claim 1 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust one or more tones of the inputted waveform.
8. The system according to claim 7 , wherein each tone of the inputted waveform comprises one or more sine waves.
9. The system according to claim 7 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust an amplitude of the one or more tones of the inputted waveform.
10. The system according to claim 1 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to:
determine an amplitude of each angularly deflected laser beam in the output laser beam;
adjust one or more parameters of the waveform inputted into the acousto-optic device for each angularly deflected laser beam.
11. The system according to claim 10 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust the amplitude of the tone of the inputted waveform for each angularly deflected laser beam.
12. The system according to claim 11 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust the frequency of the tone of the inputted waveform for each angularly deflected laser beam.
13. The system according to claim 12 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust one or more parameters of the inputted waveform to generate a plurality of angularly deflected laser beams having intensities which vary by 10% or less.
14. The system according to claim 12 , wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to adjust one or more parameters of the inputted waveform to generate a plurality of angularly deflected laser beams having intensities which vary by 5% or less.
15. The system according to claim 1 , wherein the waveform generator is an Arbitrary Waveform Generator (AWG).
16. The system according to claim 1 , wherein the plurality of angularly deflected laser beams comprise a local oscillator (LO) beam and a plurality of radiofrequency comb beams.
17. The system according to claim 1 , wherein the plurality of angularly deflected laser beams are spatially separated.
18. The system according to claim 17 , wherein the each angularly deflected laser beam at least partially overlaps with one other angularly deflected laser beam in the output laser beam.
19. The system according to claim 1 , wherein the system is a flow cytometer.