IP Library › Granted Patent US 12,203,090
Granted Patent B2
US 12,203,090 · App. 18/054,406 · Granted Jan 21, 2025

Polynucleotides and methods for transferring resistance to Asian soybean rust

Inventors: Greg Rairdan (Wilmington, DE); Karen Broglie (Landenberg, PA); Gilda Rauscher (Johnston, IA); Hendrikus Pieter Van Esse (Norfolk, GB); Jonathan D. G. Jones (Norfolk, GB); Cintia Goulart Kawashima (Norfolk, GB); Sergio Herminio Brommonschenkel (Vicosa, BR)
Assignees: Two Blades Fpondation; Corteva Agriscience LLC
C12N15/8282A01N25/00A01N65/20C07K14/415
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Quick Facts
Patent No.
US 12,203,090
App. No.
18/054,406
Granted
Jan 21, 2025
Kind
B2
Abstract

Disclosed herein are compositions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume Plant.

Claims (27)

1. A cell transformed with a recombinant DNA construct comprising a heterologous regulatory element operably linked to a polynucleotide comprising a nucleotide sequence encoding a legume-derived nucleotide binding site-leucine-rich repeat (NB-LRR) polypeptide having at least 90% amino acid sequence identity to SEQ ID NO: 2.

2. The cell of claim 1 , wherein the recombinant DNA construct encodes a legume-derived NB-LRR gene that confers resistance to a plant disease.

3. The cell of claim 1 , wherein the cell transformed with the recombinant DNA construct displays enhanced resistance to Asian soybean rust when compared to a cell not transformed with the recombinant DNA construct.

4. The cell of claim 1 , wherein the recombinant DNA construct is inserted in an expression vector.

5. The cell of claim 1 , wherein the cell is resistant to a legume crop species disease.

6. The cell of claim 5 , wherein the legume crop species disease is caused by a plant pathogen.

7. The cell of claim 6 , wherein the plant pathogen is Phakopsora pachyrhizi or Phakopsora meibomiae.

8. The cell of claim 1 , wherein the cell is a plant cell selected from the group consisting of a monocot or a dicot.

9. The cell of claim 1 , wherein the cell is a soybean cell.

10. The cell of claim 2 , wherein the legume-derived NB-LRR gene is derived from genus Cicer, Cajanus, Glycine, Lablab, Medicago, Phaseolus, Pisum, Pueraria, Trifolium or Vigna.

11. The cell of claim 10 , wherein the legume-derived NB-LRR gene is derived from:

a) Cicer arietinum, Cicer echinospermum, Cicer reticulatum or Cicer pinnatifidum;

b) Glycine arenaria, Glycine argyrea, Glycine cyrtoloba, Glycine canescens, Glycine clandestine, Glycine curvata, Glycine falcata, Glycine latifolia, Glycine microphylla, Glycine pescadrensis, Glycine stenophita, Glycine syndetica, Glycine soja, Glycine tabacina or Glycine tomentella;

c) Lablab purpureus;

d) Medicago truncatula or Medicago sativa;

e) Phaseolus vulgaris, Phaseolus lunatus, Phaseolus acutifolius or Phaseolus coccineus;

f) Pisum abyssinicum, Pisum sativum, Pisum elatius, Pisum fulvum, Pisum transcaucasium or Pisum humile;

g) Pueraria lobate;

h) Trifolium aureum or Trifolium occidentale ; and

i) Vigna unguiculata, Vigna dalzelliana, Vigna oblongifolia, Vigna parkeri, Vigna filicaulis, Vigna kirkii, Vigna luteola, Vigna radiata, Vigna trilobata, Vigna luteola , or Vigna mungo.

12. The cell of claim 2 , further comprising one or more resistance genes.

13. A method of reducing one or more symptoms of a legume plant disease, the method comprising exposing a transgenic legume crop plant transformed with a recombinant DNA construct comprising a heterologous regulatory element operably linked to a polynucleotide comprising a nucleotide sequence encoding a legume-derived nucleotide binding site-leucine-rich repeat (NB-LRR) polypeptide having at least 90% amino acid sequence identity to SEQ ID NO: 2 to the legume plant disease wherein the transgenic legume crop plant has an enhanced resistance to the plant disease.

14. The method of claim 13 , wherein the plant disease is Asian soybean rust.

15. A method of producing an Asian soybean rust resistant plant, the method comprising regenerating the transformed plant from the transformed plant cell of claim 1 .

16. The method of claim 15 , wherein the Asian soybean rust resistant plant is a legume species.

17. The method of claim 16 , where in the legume species is an alfalfa, a clover, a pea, a bean, a lentil, a lupin, a mesquite, a carob, a soybean, a peanut or a tamarind species.

18. The method of claim 17 , wherein the legume species is a soybean species.

Assignments (5)
NUNC PRO TUNC ASSIGNMENT Recorded May 30, 2024
From: E.I. DU PONT DE NEMOURS AND COMPANY
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 067571/0532 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 24, 2024
From: JONES, JONATHAN D.G.; KAWASHIMA, CINTIA G.
To: TWO BLADES FOUNDATION
Reel/Frame 067520/0808 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 24, 2024
From: VAN ESSE, HENDRIKUS PIETER
To: TWO BLADES FOUNDATION
Reel/Frame 067520/0812 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 24, 2024
From: RAIRDAN, GREG; BROGLIE, KAREN; RAUSCHER, GILDA
To: E.I. DU PONT DE NEMOURS AND COMPANY
Reel/Frame 067520/0815 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 24, 2024
From: BROMMONSCHENKEL, SERGIO HERMINIO
To: UNIVERSIDADE FEDERAL DE VICOSA
Reel/Frame 067520/0818 →
Continuity (4)
Continuation 17081582 · Oct 27, 2020
Continuation 15573149
Provisional Application 62159718 · May 11, 2015
Related Publication 20230157232A1 · May 25, 2023
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