Granted Patent
B2
US 12,195,704 · App. 18/114,051 · Granted Jan 14, 2025
Compositions for facilitating biological stain removal
Inventors:
Songtao Wu (Ann Arbor, MI); Hongfei Jia (Ann Arbor, MI); Masahiko Ishii (Okazaki, JP); Minjuan Zhang (Ann Arbor, MI)
C11D3/38618C08G18/6225C08G18/792C09D175/04C11D3/04C11D3/044C11D3/2003C11D3/201C11D3/37C11D3/386C11D3/38627C11D3/43C11D7/04C11D7/261C12Y304/24027D06L4/40D06M16/003C11D2111/18
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Abstract
A liquid bioactive composition is provided that includes an enzyme with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein the enzyme in the liquid bioactive coating material is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from a substrate.
Claims (22)
1. A composition for facilitating biological stain removal comprising a liquid bioactive coating material comprising:
a surfactant;
a compound selected from the group consisting of ammonia, a water miscible alcohol having 1 to 6 carbon atoms, and combinations thereof, and
an enzyme, said enzyme capable of degrading a biological stain component, wherein said enzyme is a thermolysin-like protease, an amylase, or a lipase.
2. The composition of claim 1 , wherein said thermolysin-like protease is an active extracellular portion of bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus.
3. The composition of claim 1 , wherein said liquid bioactive coating material includes a protease at a specific activity in excess of 20,000 U/g.
4. The composition of claim 1 , wherein the pH of said liquid bioactive coating material is greater than 5.0.
5. The composition of claim 1 , wherein said enzyme is in solution in said liquid bioactive coating material.
6. The composition of claim 1 , wherein said liquid bioactive coating material comprises said alcohol, said alcohol present at less than 0.8% by weight.
7. The composition of claim 1 , wherein said liquid bioactive coating material comprises said ammonia.
8. The composition of claim 1 , wherein said composition comprises said alcohol, and said alcohol is methanol.
9. A method of facilitating the removal of a biological stain on a substrate or a coating comprising:
providing the liquid coating material of claim 1 ;
contacting said liquid coating material to a biological stain to thereby facilitate removal of the biological stain.
10. The method of claim 9 , wherein said liquid bioactive coating material includes a protease at a specific activity in excess of 20,000 U/g.
11. The method of claim 9 , wherein the pH of the liquid bioactive coating material is greater than 5.0.
12. The method of claim 9 , wherein said enzyme is in solution in said liquid bioactive coating material.
13. The method of claim 9 , wherein said liquid bioactive coating material comprises said water miscible alcohol, said alcohol present at less than 0.8% by weight.
14. The method of claim 9 , wherein said bioactive liquid coating material comprises said ammonia.
15. The method of claim 9 , wherein said biological stain is an insect stain.
16. The method of claim 9 , wherein said biological stain is present on a substrate.
17. The method of claim 9 , wherein said substrate is glass.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 15, 2024
From: TOYOTA MOTOR ENGINEERING & MANUFACTURING NORTH AMERICA, INC.
To: TOYOTA MOTOR CORPORATION
Reel/Frame 066467/0568 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 27, 2023
From: WU, SONGTAO; JIA, HONGFEI; ZHANG, MINJUAN
To: TOYOTA MOTOR ENGINEERING & MANUFACTURING NORTH AMERICA, INC.
Reel/Frame 065367/0685 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 27, 2023
From: ISHII, MASAHIKO
To: TOYOTA MOTOR CORPORATION
Reel/Frame 065367/0804 →
Continuity (8)
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