Self-assembly of protein-based biomaterials with multiple morphologies
The present disclosure relates, in some embodiments, to a composition comprising a biomaterial. A biomaterial may comprise, for example, one or more molecules capable of self-association and/or self-assembly. In some embodiments, a biomaterial may comprise one or more polypeptides and/or proteins. A biomaterial may comprise, for example, two or more self-assembled Ultrabithorax (Ubx) protein molecules. A Ubx protein, in some embodiments, may be any wild type Drosophila melanogaster Ultrabithorax protein, including any natural or non-natural isoforms (e.g., alternative splicing isoforms). The present disclosure relates, in some embodiments, to a method of making a biomaterial comprising contacting two or more Ubx protein molecules under conditions that permit self-assembly to form a first fibril and contacting the first fibril to a second fibril.
1. A biomaterial comprising two or more self-assembled Ultrabithorax (Ubx) proteins, wherein at least one of the two or more self-assembled Ubx proteins is a fusion protein in which the Ubx protein is fused with at least one other protein, wherein the Ubx protein comprises a sequence having at least 95% identity to SEQ ID NO: 3.
2. The biomaterial of claim 1 , wherein the Ubx protein comprises the amino acid sequence of SEQ ID NO: 3.
3. The biomaterial of claim 1 , wherein the biomaterial is a fibril, a film, a fiber, a sheet, a bundle, a lattice, a scaffold or an encapsulate morphology.
4. The biomaterial of claim 1 , wherein the biomaterial comprises at least two different Ubx fusion proteins.
5. The biomaterial of claim 1 , wherein the biomaterial is a bundle that comprises at least two different Ubx fusion protein fibers.
6. The biomaterial of claim 1 , wherein the other protein is selected from the group consisting of all or part of an enzyme, an enzyme inhibitor, an antigen, an antibody, a hormone, a coagulation factor, an interferon, a cytokine, a growth factor, a differentiation factor, an osteogenic factor, a bone resorption factor, a cellular motility factor, a bactericidal factor, an antifungal factor, a chemotactic factor, a cytostatic factor, a plasma adhesive molecule, an interstitial adhesive molecule, an extracellular matrix, and a ligand binding factor.
7. The biomaterial of claim 1 , wherein the at least one other protein is a ligand.
8. The biomaterial of claim 7 , wherein the ligand is selected from the group consisting of a labeled marker, a growth factor, a transcription factor, an antibody, a cytokine, a hormone, an antibiotic, and combinations thereof.