Scalable purification method for AAV9
A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
1 . A method for separating recombinant adeno-associated virus serotype 9 (rAAV9) viral particles having packaged genomic sequences from genome-deficient adeno-associated virus serotype 9 (AAV9) capsid intermediates, said method comprising:
(a) filtering rAAV9 viral particles and genome-deficient AAV9 capsid intermediates harvested from an rAAV9 production culture, wherein the rAAV9 viral particles and the AAV9 capsid intermediates comprise an adeno-associated virus capsid serotype 9 (AAV9 capsid) comprising the amino acid sequence of SEQ ID NO: 1 or a sequence at least 99% identical to SEQ ID NO: 1, to afford a concentrated liquid suspension media comprising rAAV9 viral particles and genome-deficient AAV9 capsid intermediates,
(b) contacting the liquid suspension comprising the rAAV9 viral particles and genome-deficient AAV9 capsid intermediates from step (a) with a high-performance binding affinity resin which binds the AAV9 capsid comprising the amino acid sequence of SEQ ID NO: 1 or a sequence at least 99% identical to SEQ ID NO: 1, to afford a purified mixture containing the rAAV9 viral particles and genome-deficient AAV9 capsid intermediates;
(c) loading a suspension comprising the rAAV9 viral particles and genome-deficient AAV9 capsid intermediates from the purified mixture obtained in step (b) onto an anion exchange resin for anion exchange chromatography;
(d) equilibrating the anion exchange resin at a pH of 10.0 to 10.4; and
(e) monitoring an eluate for ultraviolet absorbance at about 260 nm (A260) and about 280 nm (A280) and collecting rAAV9 viral particles from fractions eluted after the ratio of A260 to A280 (A260/A280) reaches an inflection point, thereby separating the rAAV9 viral particles from the genome-deficient AAV9 capsid intermediates.
2 . The method of claim 1 , wherein the affinity resin comprises an anti-AAV ligand which is an anti-AAV variable domain of heavy chain (VHH) ligand, a single-domain camelid antibody, or a monoclonal antibody.
3 . The method of claim 1 , wherein the filtering of step (a) is performed through a series of depth filters.
4 . The method of claim 1 , wherein the rAAV9 production culture harvest is treated with a nuclease or a combination of nucleases to digest contaminating high molecular weight nucleic acid and to afford a mixture comprising rAAV9 viral particles and genome-deficient AAV9 capsid intermediates.
5 . The method of claim 1 , wherein the anion exchange chromatography is performed with a salt gradient of about 10 mM to about 190 mM NaCl or a salt equivalent.
6 . The method of claim 1 , wherein the anion exchange chromatography is performed with a salt gradient having an ionic strength equivalent to at least about 20 mM to about 190 mM NaCl, or a salt gradient having an ionic strength equivalent thereto, and wherein the ionic strength is calculatable using a formula I=½Σ i=1 n c i z i 2 , where ci is the molar concentration of ion i (M, mol/L), zi is the charge number of that ion, and I is taken over all ions in the solution.
7 . The method of claim 6 , wherein genome-deficient AAV9 capsid intermediates are eluted from the anion exchange resin when a salt gradient reaches an ionic strength equivalent to about 50 mM NaCl or greater, or a salt gradient having an ionic strength equivalent thereto, and wherein the ionic strength is calculatable using a formula I=½Σ i=1 n c i z i 2 , where ci is the molar concentration of ion i (M, mol/L), zi is the charge number of that ion, and I is taken over all ions in the solution.
8 . The method of claim 1 , wherein the loading of step (c) has a sample loading flow rate less than or equal to the elution flow rate of elution of step (e).
9 . The method of claim 8 , wherein the elution has an elution flow rate which is from about 10 mL/min to about 40 mL/min.
10 . The method of claim 9 , wherein the elution flow rate is about 20 mL/min.
11 . The method of claim 1 , wherein the anion exchange resin is in a column.
12 . The method of claim 11 , wherein the anion exchange resin column comprises trimethylamine and a support matrix comprising poly(glycidyl methacrylate-co-ethylene dimethacrylate).
13 . The method of claim 11 , wherein the anion exchange resin column is a monolith column, and wherein column loading of step (c), washing of step (d) and elution of step (e) occur in about 60 column volumes.
14 . The method of claim 1 , wherein the rAAV9 viral particles and genome-deficient AAV9 capsid intermediates had been purified from production system contaminants using affinity capture, wherein the contaminants comprise non-AAV viral materials, cellular proteinaceous materials, and nucleic acid materials.
15 . The method of claim 3 , wherein the series of depth filters comprise filters of 0.2 μm or greater pore size.
16 . The method of claim 3 , wherein the series of depth filters comprise filters in the range of about 0.045 μm to about 0.2 μm pore size.
17 . The method of claim 1 , wherein the pH in (d) is 10.2.
18 . The method of claim 1 , further comprising eluting rAAV9 viral particles at a pH of 10.2 in step (e).
19 . A method for separating recombinant adeno-associated virus 9 (rAAV9) viral particles having packaged genomic sequences from genome-deficient rAAV9 capsid intermediates, said method comprising:
(a) purifying a mixture comprising recombinant AAV9 viral particles and rAAV9 capsid intermediates from production system contaminants using an AAV-specific antibody-based affinity capture resin;
(b) subjecting the mixture comprising recombinant AAV9 viral particles and rAAV9 capsid intermediates to fast performance liquid chromatography, wherein the recombinant AAV9 viral particles and rAAV9 capsid intermediates are bound to a strong anion exchange resin equilibrated at a pH of 10.0 to 10.4;
(c) applying an increasing salt concentration gradient to the loaded anion exchange resin while monitoring eluate for ultraviolet absorbance at 260 nm (A260) and 280 nm (A280); and
(d) collecting the recombinant AAV9 viral particles from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.
20 . The method according to claim 19 , wherein the inflection point is when the ratio of A260/A280 changes from less than 1 to greater than 1.
21 . The method according to claim 19 , wherein the salt gradient has an ionic strength equivalent to at least 10 mM to 190 mM NaCl, and/or wherein the rAAV9 capsid intermediates are eluted from the anion exchange resin when the salt gradient reaches an ionic strength equivalent to 50 mM NaCl or greater.
22 . The method according to claim 19 , wherein the mixture of (b) comprising the recombinant AAV9 viral particles and rAAV9 capsid intermediates contains less than 10% contamination from viral and cellular proteinaceous and nucleic acid materials, optionally wherein the mixture of (b) is at least 95% purified from viral and cellular proteinaceous and nucleic acid materials.
23 . The method according to claim 19 , wherein the method has a sample loading flow rate less than or equal to the elution flow rate.
24 . The method according to claim 19 , wherein the anion exchange resin is in a column.
25 . The method according to claim 19 , said method comprising, in that order:
(a) purifying a mixture comprising recombinant AAV9 viral particles and rAAV9 capsid intermediates from production system contaminants using affinity capture;
(b) mixing a suspension comprising recombinant AAV9 viral particles and rAAV9 capsid intermediates with a buffer comprising 20 mM Bis-Tris propane (BTP) and having a pH of 10.0 to 10.4;
(c) loading the suspension of (b) onto a strong anion exchange resin equilibrated at a pH of 10.0 to 10.4 and subjecting the mixture comprising recombinant AAV9 viral particles and rAAV9 capsid intermediates to fast performance liquid chromatography, wherein the recombinant AAV9 viral particles and rAAV9 capsid intermediates are bound to the strong anion exchange resin;
(d) washing the loaded anion exchange resin with a buffer comprising 20 mM BTP and 10 mM NaCl, and having a pH of 10.0 to 10.4,
(e) applying an increasing salt concentration gradient to the loaded anion exchange resin while monitoring eluate for ultraviolet absorbance at 260 nm (A260) and 280 nm (A280); and
(f) collecting the recombinant AAV9 viral particles from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point,
wherein the salt gradient is from 10 mM to 190 mM NaCl or a salt equivalent; and wherein the collected recombinant AAV9 viral particles from step (f) are at least 90% purified from rAAV9 capsid intermediates.
26 . The method according to claim 25 , wherein the recombinant AAV9 viral particles and rAAV9 capsid intermediates of step (b) have been affinity purified at a high salt concentration.
27 . The method according to claim 25 , wherein the anion exchange resin is a quaternary amine ion exchange resin, optionally wherein the anion exchange resin comprises trimethylamine and a support matrix comprising poly(glycidyl methacrylate-co-ethylene dimethacrylate).
28 . The method according to claim 25 , wherein the buffer of (b) is further admixed with NaCl to a final concentration of 10 mM in order to form or prepare the buffer of (d).
29 . The method according to claim 25 , wherein the elution gradient is from 1% to 19% of a buffer comprising 1 M NaCl, 20 mM Bis-Tris-Propane (BTP) and having a pH of 10.0 to 10.4.
30 . The method according to claim 25 , wherein the anion exchange resin is a monolith column and wherein column loading, washing and elution occur in 54 to 66 column volumes.
31 . The method according to claim 25 , wherein the elution flow rate is from 10 mL/min to 40 mL/min, optionally wherein the elution flow rate is 20 mL/min.
32 . The method according to claim 19 , said method further comprising:
(e′) prior to the step of (b), forming a loading suspension comprising:
recombinant AAV9 viral particles and rAAV9 capsid intermediates which have been purified to remove non-AAV materials from an AAV producer cell culture in which the particles and intermediates were generated; and a buffer comprising 20 mM BTP at a pH of 10.0 to 10.4; and
(f′) after the step of (b), washing the loaded anion exchange resin with a buffer comprising 10 mM NaCl and 20 mM BTP with a pH of 10.0 to 10.4,
wherein the strong anion exchange resin is in a vessel having an inlet for flow of a suspension and/or solution and an outlet permitting flow of eluate from the vessel and wherein the salt gradient ranges from 10 mM to 190 mM NaCl, inclusive of the endpoints, or an equivalent.
33 . The method according to claim 32 , wherein the pH of the suspension of (e′) and (f′) is 10.2 and the collected recombinant AAV9 viral particles are at least 90% purified from rAAV9 capsid intermediates.
34 . The method according to claim 32 , wherein the average yield of recombinant AAV9 viral particles is at least 40% to 70% as measured by genome copy (GC) titer.
35 . The method according to claim 32 , wherein the producer cell culture is selected from a mammalian cell culture, a bacterial cell culture, and an insect cell culture, wherein said producer cells comprise at least (i) nucleic acid sequence encoding an rAAV9 capsid operably linked to sequences which direct expression of the rAAV9 capsid in the producer cells; (ii) a nucleic acid sequence comprising AAV inverted terminal repeat sequences and genomic transgene sequences for packaging into the rAAV9 capsid; and (iii) functional AAV rep sequences operably linked to sequences which direct expression thereof in the producer cells.
36 . The method according to claim 32 , wherein material harvested from the cell culture is applied to an affinity resin to separate contaminants from recombinant AAV9 viral particles and rAAV9 capsid intermediates, optionally wherein the affinity resin separation comprises:
(i) equilibrating the affinity resin with a buffer which comprises 200 mM to 600 mM NaCl and a neutral pH prior to applying the material to the affinity resin;
(ii) washing the loaded resin of (i) with a buffer which comprises 800 mM NaCl to 1200 mM NaCl and a neutral pH;
(iii) washing the buffer-washed resin of (ii) with the buffer of (i) to reduce salt concentration;
(iv) washing the affinity resin of (iii) with a buffer which comprises 200 mM to 600 mM NaCl, 20 mM Sodium Citrate, at a pH of 2.4 to 3; and
(v) collecting the eluate of (iv) which comprises the recombinant AAV9 viral particles and the rAAV9 capsid intermediates fraction for loading onto the anion exchange resin.
37 . The method according to claim 36 , wherein the neutral pH is 7.5, and/or wherein in (iv), the pH is 2.5, and/or wherein the buffer of (i) and/or the buffer of (iv), independently have 400 mM NaCl.