Droplet forming devices and system with differential surface properties
Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
1. A method for producing droplets comprising:
A) providing a system comprising:
a) a device comprising:
i) a first channel having a first depth, a first width, a first proximal end, a first distal end, and a first surface comprising a material;
ii) a shelf region having a second depth and a second width, wherein the second width is greater than the first width, wherein the shelf region comprises a hydrophobic coating different from the material of the first surface, and wherein the first distal end is in fluid communication with the shelf region;
iii) a first reservoir in fluid communication with the first proximal end and comprising at least a portion of the first liquid; and
iv) a collection reservoir configured to collect the droplets and comprising at least one wall that forms a step fluidically connected to the shelf region; and
b) a second liquid contained in the shelf region and the collection reservoir, wherein the first liquid and the second liquid are immiscible; and
B) flowing the first liquid to the shelf region, wherein the first liquid expands at the shelf region and the step to produce droplets of the first liquid, wherein the droplets are dispersed in the second liquid in the collection reservoir.
2. The method of claim 1 , wherein prior to A), the first liquid comprises one or more particles, and the first reservoir comprises at least a portion of the first liquid and the one or more particles.
3. The method of claim 2 , wherein the one or more particles are beads.
4. The method of claim 2 , wherein B) produces droplets having a single particle.
5. The method of claim 1 , wherein the first liquid is aqueous or miscible with water.
6. The method of claim 1 , wherein the device further comprises a second channel having a second channel depth, a second channel width, a second channel proximal end, and a second channel distal end, wherein the second channel intersects the first channel between the first proximal and first distal ends.
7. The method of claim 6 , wherein the device further comprises a second reservoir in fluid communication with the second channel proximal end and comprises at least one portion of the first liquid, and, wherein, prior to B), the portion of the first liquid in the first channel and the portion of the first liquid in the second channel combine at the intersection of the first channel and second channel to form the first liquid.
8. The method of claim 7 , wherein the system further comprises a controller operatively coupled to transport the portion of the first liquid in the first reservoir and the portion of first liquid in the second reservoir to the intersection.
9. The method of claim 6 , wherein the second channel comprises a third liquid, wherein the third liquid combines with the first liquid at the intersection of the first channel and the second channel prior to B), wherein B) produces droplets of the combined first liquid and third liquid.
10. The method of claim 9 , wherein the third liquid is aqueous or miscible with water.
11. The method of claim 10 , wherein in A), the first or third liquid comprises one or more biological particles and the first or third liquid comprises one or more beads, and the droplets produced in B) comprise the first liquid, the third liquid, a single bead, and a single biological particle.
12. A method for producing droplets comprising:
A) providing a system comprising:
a) a device comprising:
i) a plurality of first channels each having a first depth, a first width, a first proximal end, a first distal end, and a first surface comprising a material;
ii) a plurality of second channels each having a second channel depth, a second channel width, a second channel proximal end, and a second channel distal end;
iii) a plurality of shelf regions each having a second depth and a second width, wherein each of the first distal ends of the plurality of first channels is in fluid communication with a corresponding one of the plurality of shelf regions, wherein each of the second widths is greater than the corresponding first width the plurality of first channels, wherein each of the plurality of shelf regions comprises a hydrophobic coating different from the material of the first surfaces of the plurality of first channels;
iv) a first reservoir in fluid communication with at least one of the first proximal ends of the plurality of first channels; and
v) a collection reservoir configured to collect the droplets and comprising at least one wall that forms a plurality of steps each fluidically connected to a corresponding one of the plurality of shelf regions;
wherein each of the plurality of second channels intersects a corresponding one of the plurality of first channels between the first proximal and first distal ends of the corresponding one of the plurality of first channels;
b) a first liquid;
c) a second liquid; and
d) a third liquid;
wherein the first liquid and the third liquid are not miscible with the second liquid;
wherein each of the plurality of first channels comprises the first liquid, each of the plurality of second channels comprises the third liquid, and the plurality of shelf regions and the collection reservoir comprise the second liquid;
B) combining the first liquid and the third liquid at the intersections of the at least one of the plurality of first channels and the at least one of the plurality of second channels;
C) flowing the combined first and third liquids to the one of the plurality of shelf regions, wherein the combined first liquid and third liquid expands at the one of the plurality of shelf regions and the plurality of steps to produce droplets of the combined first liquid and third liquid, wherein the droplets are dispersed in the second liquid in the collection reservoir.
13. The method of claim 12 , wherein prior to A), the first liquid or third liquid comprises one or more particles.
14. The method of claim 13 , wherein the one or more particles are beads.
15. The method of claim 13 , wherein C) produces droplets having a single particle.
16. The method of claim 14 , wherein in A), the first or third liquid comprises one or more biological particles, and the droplets produced in C) comprise the first liquid, the third liquid, a single bead, and a single biological particle.
17. The method of claim 12 , wherein the first liquid is aqueous or miscible with water.
18. The method of claim 12 , wherein the third liquid is aqueous or miscible with water.
19. The method of claim 12 , wherein the device further comprises a second reservoir in fluid communication with at least one of the second channel proximal ends of the plurality of second channels and comprises the third liquid.
20. The method of claim 19 , wherein the system further comprises a controller operatively coupled to transport the first liquid in the first reservoir and the third liquid in the second reservoir to one of the intersections of the plurality of first channels and the plurality of second channels.