Method and apparatus for tissue grafting and copying
Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and/or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and/or viability of the harvested tissue.
1 . An apparatus comprising:
a first plurality of hollow tubes, each hollow tube of the first plurality of hollow tubes being configured to be inserted into a biological tissue at a donor site to remove a corresponding portion of the biological tissue therefrom and to retain the corresponding portion of the biological tissue therein;
a second plurality of hollow tubes, each hollow tube of the second plurality of hollow tubes being configured to be inserted into the biological tissue at the donor site to remove a corresponding portion of the biological tissue therefrom and to retain the corresponding portion of the biological tissue therein;
one or more linear actuators configured to move the first plurality of hollow tubes and the second plurality of hollow tubes; and
wherein the first plurality of hollow tubes are independently translatable from the second plurality of hollow tubes.
2 . The apparatus of claim 1 , further comprising a substrate including one or more holes therethrough;
wherein the first plurality of hollow tubes and the second plurality of hollow tubes are coupled to the substrate;
wherein the one or more linear actuators are coupled to the substrate; and
wherein the first plurality of hollow tubes and the second plurality of hollow tubes are configured to pass through the one or more holes of the substrate.
3 . The apparatus of claim 2 , wherein each hollow tube of the first plurality of hollow tubes is configured to be raised above a lower surface of the substrate; and
wherein each hollow tube of the second plurality of hollow tubes is configured to be raised above the lower surface of the substrate.
4 . The apparatus of claim 3 , wherein the substrate is configured to be placed directly on a surface of the donor site to provide mechanical stabilization and to reduce movement of the biological tissue at the donor site.
5 . The apparatus of claim 1 , wherein a width of a lumen of each hollow tube of the first plurality and second plurality of hollow tubes is 1 mm or less; and
wherein the biological tissue is skin tissue.
6 . The apparatus of claim 1 , further comprising a plurality of pins, each pin of the plurality of pins being provided at least partially within a respective hollow tube of the first plurality of hollow tubes and the second plurality of hollow tubes.
7 . The apparatus of claim 6 , wherein each pin of the plurality of pins is configured to facilitate a removal of the corresponding portion of the biological tissue from the respective hollow tube of the first plurality and the second plurality of hollow tubes.
8 . The apparatus of claim 1 , wherein at least one corresponding portion of the biological tissue is a cylinder.
9 . An apparatus comprising:
a first plurality of hollow tubes, each hollow tube of the first plurality of hollow tubes being configured to be inserted into a biological tissue at a donor site to remove a corresponding portion of the biological tissue therefrom and to retain the corresponding portion of the biological tissue therein;
a second plurality of hollow tubes, each hollow tube of the second plurality of hollow tubes being configured to be inserted into the biological tissue at the donor site to remove a corresponding portion of the biological tissue therefrom and to retain the corresponding portion of the biological tissue therein;
wherein the first plurality of hollow tubes are independently translatable from the second plurality of hollow tubes; and
wherein a distal end of each hollow tube of the first plurality and second plurality of hollow tubes includes:
a first extension that defines a first bevel on an exterior surface of the respective hollow tube; and
a second extension that defines a second bevel on an exterior surface of the respective hollow tube.
10 . An apparatus comprising:
a substrate including one or more holes therethrough;
a first hollow tube that is configured to be inserted into a biological tissue at a donor site to remove a first portion of the biological tissue therefrom, the first hollow tube being coupled to the substrate;
a second hollow tube that is configured to be inserted into the biological tissue at the donor site to remove a second portion of the biological tissue therefrom, the second hollow tube being coupled to the substrate;
wherein the first hollow tube and the second hollow tube are configured to pass through the one or more holes of the substrate;
wherein the first hollow tube is independently translatable from the second hollow tube;
wherein the first hollow tube and the second hollow tube are configured to be raised above a lower surface of the substrate; and
wherein the first hollow tube is slidably attached to the substrate and the second hollow tube is slideably attached to the substrate.
11 . The apparatus of claim 10 , wherein the substrate is configured to be placed directly on a surface of the donor site to provide mechanical stabilization and to reduce movement of the biological tissue at the donor site.
12 . The apparatus of claim 10 , wherein a width of a lumen of each hollow tube of the first hollow tube and the second hollow tube is 1 mm or less; and
wherein the biological tissue is skin tissue.
13 . The apparatus of claim 10 , wherein at least one corresponding portion of the biological tissue is a cylinder.
14 . The apparatus of claim 10 , further comprising a plurality of pins, each pin of the plurality of pins being provided at least partially within a respective hollow tube of the first hollow tube and the second hollow tube.
15 . The apparatus of claim 14 , wherein each pin of the plurality of pins is configured to facilitate a removal of the corresponding portion of the biological tissue from the respective hollow tube of the first hollow tube and the second hollow tube.
16 . An apparatus comprising:
a substrate including one or more holes therethrough;
a first hollow tube that is configured to be inserted into a biological tissue at a donor site to remove a first portion of the biological tissue therefrom, the first hollow tube being coupled to the substrate;
a second hollow tube that is configured to be inserted into the biological tissue at the donor site to remove a second portion of the biological tissue therefrom, the second hollow tube being coupled to the substrate;
one or more linear actuators configured to move the first hollow tube and the second hollow tube;
wherein the first hollow tube and the second hollow tube are configured to pass through the one or more holes of the substrate;
wherein the first hollow tube is independently translatable from the second hollow tube; and
wherein the first hollow tube and the second hollow tube are configured to be raised above a lower surface of the substrate.
17 . The apparatus of claim 16 , wherein the substrate is configured to be placed directly on a surface of the donor site to provide mechanical stabilization and to reduce movement of the biological tissue at the donor site.
18 . The apparatus of claim 16 , wherein a width of a lumen of each hollow tube of the first hollow tube and the second hollow tube is 1 mm or less; and
wherein the biological tissue is skin tissue.
19 . The apparatus of claim 16 , further comprising one or more linear actuators configured to move the first hollow tube and the second hollow tube.
20 . The apparatus of claim 16 , further comprising a plurality of pins, each pin of the plurality of pins being provided at least partially within a respective hollow tube of the first hollow tube and the second hollow tube.
21 . The apparatus of claim 20 , wherein each pin of the plurality of pins is configured to facilitate a removal of the corresponding portion of the biological tissue from the respective hollow tube of the first hollow tube and the second hollow tube.