Granted Patent
B2
US 12,467,047 · App. 18/513,267 · Granted Nov 11, 2025
Accelerated directed evolution of microbial consortia for the development of desirable plant phenotypic traits
Assignee:
BIOCONSORTIA, INC.
C12N15/1058A01H3/00A01N63/00A01N63/20A01N63/22A01N63/27A01N63/28A01N63/30A01N63/36C12N1/20G06F3/04817G06F3/0488G06Q20/12G06Q20/322G06Q30/06G06Q30/0641
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Abstract
The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants.
Claims (25)
1 . A method for modifying the microbial community in a soil, said method comprising:
(a) growing a plurality of plants in a growth medium, in the presence of a first microbial community;
(b) selecting at least one plant from said plurality of plants, following step (a), based upon a beneficial phenotypic trait exhibited by the plant relative to other plants of said plurality;
(c) acquiring a second microbial community from the at least one plant selected in step (b);
(d) repeating steps (a) to (c) in an iterative manner, wherein the second microbial community acquired in step (c) is used as an inoculum in step (a) of any successive repeat;
(e) acquiring a microbial community that is associated with promoting the development of a beneficial phenotypic plant trait and placing said microbial community in a medium;
(f) separating the microbial community from the medium after a period of time to provide one or more microbial composition; and
(g) applying the one or more microbial composition of (f) to a soil.
2 . The method according to claim 1 , wherein the microbial composition of (f) is formulated as a liquid.
3 . The method according to claim 1 , wherein the microbial composition of (f) is formulated as a solid.
4 . The method according to claim 1 , further comprising placing a plant or plant part in the soil of (g).
5 . The method according to claim 1 , wherein the composition comprises an agriculturally acceptable auxiliary compound.
6 . The method according to claim 1 , wherein the plurality of plants are members of a type of crop selected from the group consisting of: cereal, oilseed, fruit, vegetable, herb, legume, root, sugar, spice, forage, fiber, biofuel, and root.
7 . The method according to claim 1 , wherein the plurality of plants are legumes.
8 . The method according to claim 1 , wherein the plurality of plants are cotton plants.
9 . The method according to claim 1 , wherein the plurality of plants are soybean plants.
10 . The method according to claim 1 , wherein the plurality of plants are Zea mays plants.
11 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is from a root, stem, and/or foliar tissue from the at least one plant.
12 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is not acquired from soil surrounding the at least one plant.
13 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is not acquired from a root nodule from the at least one plant.
14 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is acquired from above-ground tissue from the at least one plant.
15 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is acquired from below-ground tissue from the at least one plant.
16 . The method according to claim 1 , wherein the second microbial community acquired in step (c) is fractionated or normalised to adjust the ratio of microbial organisms present therein.
17 . The method according to claim 1 , wherein the at least one plant selected in step (b) exhibits a superior beneficial phenotypic trait, relative to other plants of said plurality, selected from the group consisting of: growth rate, height, weight, production of metabolite, production of protein, production of carbohydrate, production of oil, leaf size, stem size, general health, survivability, speed of seed germination, quantity of biomass produced, root growth, leaf growth, shoot growth, grain yield, fibre yield, oil yield, seed yield, stress response, insect resistance, disease resistance, nematode resistance, and any combination and/or plurality thereof.
18 . The method according to claim 1 , wherein said first microbial community in step a) is obtained from a pre-existing microbial collection, database, library, or depository.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 8, 2023
From: WIGLEY, PETER; GEORGE, CAROLINE; TURNER, SUSAN
To: BIODISCOVERY NEW ZEALAND LIMITED
Reel/Frame 065815/0853 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 8, 2023
From: BIODISCOVERY NEW ZEALAND LTD
To: BIOCONSORTIA, INC.
Reel/Frame 065816/0011 →
Priority Claims (1)
NZ 602532
· Sep 19, 2012
· national
Continuity (9)
Continuation In Part
PCTNZ2013000171
· Sep 19, 2013
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