Method for stabilizing extracellular vesicles
The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.
1. A method of stabilizing an extracellular vesicle, the method comprising:
mixing the extracellular vesicle-containing sample with a first chelating agent, to obtain a first mixture;
separating the first mixture into (i) a liquid portion comprising the first chelating agent and (ii) a precipitation portion comprising the extracellular vesicles;
removing the liquid portion;
recovering the precipitation portion;
mixing the precipitation portion with a second chelating agent in a first solution to obtain a second mixture;
freeze-drying the second mixture to obtain a freeze-dried extracellular vesicle; and
dissolving the freeze-dried extracellular vesicle in a second solution.
2. The method of claim 1 , wherein a concentration of the first chelating agent in the mixing is from 20 to 200 mM.
3. The method of claim 1 , wherein a concentration of the first chelating agent in the mixing is from 40 to 200 mM.
4. The method of claim 1 , wherein a concentration of the first chelating agent in the mixing is from 50 to 200 mM.
5. The method of claim 1 , wherein the mixing the precipitation portion with a second chelating agent in a first solution to obtain a second mixture is carried out by mixing the precipitation portion with the chelating agent in the presence of a saccharide.
6. The method of claim 5 , wherein a concentration of the saccharide in the mixing is from 2.5 to 100 mg/mL.
7. The method according to claim 1 , wherein the extracellular vesicle is an exosome.
8. The method of claim 1 , wherein the extracellular vesicle-containing sample is a body fluid or a culture supernatant.
9. The method of claim 1 , wherein the extracellular vesicle-containing sample is a blood sample.
10. The method of claim 5 , wherein a concentration of the second chelating agent is from 1 to 200 mM per 10 mg/mL of the saccharide.