Granted Patent
B2
US 12,650,436 · App. 19/444,100 · Granted Jun 9, 2026
Detection of vitamins A and E by tandem mass spectrometry
Assignee:
Quest Diagnostics Investments LLC
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Abstract
A method for determining a combined amount of β-tocopherol and γ-tocopherol in a sample by tandem mass spectrometry includes subjecting the sample to ionization under conditions suitable to produce one or more ions from the β-tocopherol and γ-tocopherol detectable by mass spectrometry; and determining the amount of one or more of the ions by tandem mass spectrometry. Tandem mass spectrometry methods of this disclosure involve fragmenting β-tocopherol and γ-tocopherol ions having a mass to charge ratio of about 416.35±0.80 into one or more fragment ions including a fragment ion having a mass to charge ratio of about 151.00±0.80, wherein the amount of the one or more ions determined is related to the combined amount of β-tocopherol and γ-tocopherol in the sample.
Claims (13)
1 . A kit for a quantitative assay of simultaneously detecting vitamin A and vitamin E in a sample, the kit comprising an internal standard for analyzing vitamin A and an internal standard for analyzing vitamin E using mass spectrometry.
2 . The kit of claim 1 , wherein the internal standard for analyzing vitamin A is isotopically labeled vitamin A.
3 . The kit of claim 2 , wherein the isotopically labeled vitamin A is labeled with one or more isotopes selected from: deuterium ( 2 H), 13 C, and 15 N.
4 . The kit of claim 3 , wherein the isotopically labeled vitamin A is labeled with at least one deuterium.
5 . The kit of claim 4 , wherein the isotopically labeled vitamin A is d5-vitamin A.
6 . The kit of claim 5 , wherein the isotopically labeled vitamin A is d5-retinol.
7 . The kit of claim 1 , wherein the internal standard for analyzing vitamin A is present in an amount sufficient for performing at least one assay for vitamin A.
8 . The kit of claim 1 , wherein the internal standard for analyzing vitamin E is isotopically labeled vitamin E.
9 . The kit of claim 8 , wherein the isotopically labeled vitamin E is labeled with one or more isotopes selected from: deuterium ( 2 H), 13 C, and 15 N.
10 . The kit of claim 9 , wherein the isotopically labeled vitamin E is labeled with at least one deuterium.
11 . The kit of claim 10 , wherein the isotopically labeled vitamin E is d9-α-tocopherol.
12 . The kit of claim 1 , wherein the vitamin E is α-tocopherol or combined β-tocopherol and γ-tocopherol.
13 . The kit of claim 1 , wherein the internal standard for analyzing vitamin E is present in an amount sufficient for performing at least one assay for vitamin E.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 9, 2026
From: CHAN, SUM; JIANG, QIBO
To: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
Reel/Frame 073423/0725 →
CHANGE OF NAME
Recorded Jan 9, 2026
From: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
To: QUEST DIAGNOSTICS INVESTMENTS LLC
Reel/Frame 074304/0592 →
Continuity (7)
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