B1k chain of laminin and methods of use
Antibodies directed against laminin B1k are provided.
1. An isolated monoclonal antibody that binds to domain VI of SEQ ID NO:2.
2. The An isolated monoclonal antibody that binds to domain III of SEQ ID NO:2.
3. An isolated monoclonal antibody that binds to domain II of SEQ ID NO:2.
4. An isolated monoclonal antibody that binds to domain I of SEQ ID NO:2.
5. An isolated monoclonal antibody that binds to domain alpha of SEQ ID NO:2.
6. An isolated monoclonal antibody that binds to EGF domains 2, 3, 4, 5 or 6 of SEQ ID NO:2.
7. The monoclonal antibody of claim 1 wherein the antibody is a mouse monoclonal antibody.
8. A method for making a domain-specific antibody, the method comprising:
immunizing an experimental animal with an immunogen comprising an isolated polypeptide that consists of an amino acid sequence of a domain of SEQ ID NO:2 or a fragment of the domain, wherein the domain is selected from the group consisting of:
(a) domain VI,
(b) domain III/V,
(c) domain II,
(d) domain I,
(e) domain alpha,
(f) EGF domain 1,
(g) EGF domain 2,
(h) EGE domain 3,
(i) EGF domain 4,
(j) EGE domain 5 and,
(k) EGF domain 6; and
preparing, from the experimental animal, an antibody that binds to the immunogen.
9. The method of claim 8 , wherein the immunogen is a polypeptide consisting of domain VI of SEQ ID NO:2 or an immunogenic fragment thereof.
10. The method of claim 8 , wherein the immunogen is a polypeptide consisting of domain III/V of SEQ ID NO:2 or an immunogenic fragment thereof.
11. The method of claim 8 , wherein the immunogen is a polypeptide consisting of domain II of SEQ ID NO:2 or an antigenic fragment thereof.
12. The method of claim 8 , wherein the immunogen is a polypeptide consisting of domain I of SEQ ID NO:2 or an antigenic fragment thereof.
13. The method of claim 8 , wherein the immunogen is a polypeptide consisting of domain alpha of SEQ ID NO:2 or an antigenic fragment thereof.
14. The method of claim 8 , wherein the immunogen is a polypeptide consisting of any one of EGF domains 1, 2, 3, 4, 5 or 6 SEQ ID NO:2 or an antigenic fragment thereof.
15. The method of any one of claims 8 - 14 , wherein the animal is a mouse.
16. The method of any one of claims 8 - 14 , wherein the antibody is a monoclonal antibody.
17. The monoclonal antibody of claim 2 wherein the antibody is a mouse monoclonal antibody.
18. The monoclonal antibody of claim 3 wherein the antibody is a mouse monoclonal antibody.
19. The monoclonal antibody of claim 4 wherein the antibody is a mouse monoclonal antibody.
20. The monoclonal antibody of claim 5 wherein the antibody is a mouse monoclonal antibody.
21. The monoclonal antibody of claim 6 wherein the antibody is a mouse monoclonal antibody.
22. The monoclonal antibody of claim 7 wherein the antibody is a mouse monoclonal antibody.
23. A substantially pure preparation comprising a monoclonal antibody according to claim 1 .
24. A substantially pure preparation comprising a monoclonal antibody according to claim 2 .
25. A substantially pure preparation comprising a monoclonal antibody according to claim 3 .
26. A substantially pure preparation comprising a monoclonal antibody according to claim 4 .
27. A substantially pure preparation comprising a monoclonal antibody according to claim 5 .
28. A substantially pure preparation comprising a monoclonal antibody according to claim 6 .
29. A substantially pure preparation comprising a monoclonal antibody according to claim 7 .