Nanolipidic particles
Nanolipidic Particles (NLPs) having average mean diameters of 1 nm to 20 nm are made from a precursor solution. NLPs can be loaded with a desired passenger molecule. Assemblies of these particles, called NLP assemblies, result in a vehicle population of a desired size. Single application or multifunction NLP assemblies are made from the loaded NLPs and range in size from about 30 to about 200 nm. A method of using preloaded NLPs to make larger carrier vehicles or a mixed population provides increased encapsulation efficiency. NLPs have application in the cosmetics, pharmaceutical, and food and beverage industries.
1. A method for making a nanolipidic particle (NLP) population, comprising the steps of:
a. adding a passenger molecule selected from the group consisting of lipophilic and amphipathic passenger molecules to a monophasic precursor solution to form a loaded vehicle population;
b. diluting said loaded vehicle population with ethanol to form an NLP loaded particle population, wherein said diluting of said NLP loaded particle population with said ethanol solvent is at a ratio of about 1 part loaded vehicle population to about 20 parts solvent to about 1 part loaded vehicle population to about 0.5 parts solvent, and
c. adding an aliquot of said NLP loaded particle population to an aqueous solvent to form an NLP assembly population having a mean particle diameter from about 30 to 200 nm, and wherein the concentration of said ethanol solvent in said NLPs is from about 0.5% to about 14%.
2. The method of claim 1 , wherein said diluting of said NLP loaded particle population with said ethanol solvent is at a ratio of about 1 part loaded vehicle population to about 10 parts solvent to about 1 part loaded vehicle population to about 0.5 part solvent.
3. The method of claim 1 , wherein said aqueous solvent further comprises a water-soluble passenger molecule.
4. The method of claim 1 , wherein two or more different lipophilic or amphipathic passenger molecules are added to said precursor solution to form a loaded vehicle population wherein said vehicles encapsulate admixed passenger molecules.
5. The method of claim 1 , wherein said precursor solution comprises phospholipids selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI) and mixtures thereof.
6. The method of claim 5 , wherein said phospholipids consist of a mixture of phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
7. The method of claim 5 , wherein said phospholipids consist of a mixture of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
8. The method of claim 7 , wherein the ratio of said phospholipids in said mixture is PC:PE:PA:PI of 6.5:2.5:0.7:0.3.
9. The method of claim 1 , further comprising making one or more additional dilutions of said NLP loaded particle population with ethanol prior to adding an aliquot to said aqueous solvent.
10. The method of claim 9 , wherein the number of loaded NLPs per unit volume is selected by making said additional dilutions.
11. A method for making a nanolipidic particle (NLP) population, comprising the steps of:
a. diluting a monophasic precursor solution with ethanol to form an NLP particle population;
b. adding a passenger molecule selected from the group consisting of lipophilic and amphipathic passenger molecules to the solvent diluted precursor solution to form an NLP loaded particle population, wherein said diluting of said NLP loaded particle population with said ethanol solvent is at a ratio of about 1 part loaded vehicle population to about 20 parts solvent to about 1 part loaded vehicle population to about 0.5 parts solvent, and
c. adding an aliquot of said NLP loaded particle population to an aqueous solvent to form an NLP assembly population having a mean particle diameter from about 30 to 200 nm; and wherein the concentration of said ethanol solvent in said NLPs is from about 0.5% to about 14%.
12. The method of claim 11 , wherein said diluting of said NLP loaded particle population with said ethanol solvent is at a ratio of about 1 part loaded vehicle population to about 10 parts solvent to about 1 part loaded vehicle population to about 0.5 part solvent.
13. The method of claim 11 , wherein said aqueous solvent further comprises a water-soluble passenger molecule.
14. The method of claim 11 , wherein two or more different lipophilic or amphipathic passenger molecules are added to said solvent-diluted precursor solution to form a loaded vehicle population wherein said vehicles encapsulate admixed passenger molecules.
15. The method of claim 11 , wherein said precursor solution comprises phospholipids selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI) and mixtures thereof.
16. The method of claim 15 , wherein said phospholipids consist of a mixture of phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
17. The method of claim 15 , wherein said phospholipids consist of a mixture of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
18. The method of claim 17 , wherein the ratio of said phospholipids in said mixture is PC:PE:PA:PI of 6.5:2.5:0.7:0.3.
19. The method of claim 11 , further comprising making one or more additional dilutions of said NLP loaded particle population with ethanol prior to adding an aliquot to said aqueous solvent.
20. The method of claim 19 , wherein the number of loaded NLPs per unit volume is selected by making said additional dilutions.