Methods for identifying compounds that modulate sensory signaling through the taste cell specific G-protein coupled receptor GPCR-B4
The invention provides isolated nucleic acid and amino acid sequences of sensory cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sensory cell specific G-protein coupled receptors.
1. A method for identifying a compound that modulates sensory signaling in sensory cells, the method comprising the steps of:
(i) contacting the compound with a polypeptide comprising an extracellular domain of a sensory transduction G-protein coupled receptor, the extracellular domain comprising greater than about 90% amino acid sequence identity to the extracellular domain of SEQ ID NO:1 or SEQ ID NO:2; and
(ii) determining the functional effect of the compound upon the extracellular domain.
2. The method of claim 1 , wherein the extracellular domain is linked to a solid phase.
3. The method of claim 1 , wherein the functional effect is a chemical effect.
4. The method of claim 1 , wherein the functional effect is determined by measuring binding of the compound to the extracellular domain.
5. The method of claim 1 , wherein the polypeptide is recombinant.
6. The method of claim 1 , Wherein the polypeptide is from a rat, a mouse, or a human.
7. The method of claim 1 , wherein the polypeptide is expressed in a cell or cell membrane,
8. The method of claim 1 , wherein the functional effect is a physical effect.
9. The method of claim 1 , wherein the extracellular domain of the contacted polypeptide comprises greater than about 90% amino acid sequence identity to the extracellular domain of SEQ ID NO:1.
10. The method of claim 1 , wherein the extracellular domain of the contacted polypeptide comprises greater than about 95% amino acid sequence identity to the extracellular domain of SEQ ID NO:1.
11. The method of claim 1 , wherein the extracellular domain of the contacted polypeptide comprises greater than about 90% amino acid sequence identity to the extracellular domain of SEQ ID NO:2.
12. The method of claim 1 , wherein the extracellular domain of the contacted polypeptide comprises greater than about 95% amino acid sequence identity to the extracellular domain of SEQ ID NO:2.