Subtypes of humanized antibody against interleukin-6 receptor
An antibody subtype (1) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH 2 (447), and an antibody subtype (2) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which both C-terminals of the heavy chain are Pro-NH 2 (447), and a pharmaceutical composition comprising them.
1. A method of inhibiting biological activity of interleukin-6 receptor (IL-6R) with an antibody subtype against IL-6R, comprising contacting the IL-6R with the antibody subtype, wherein the antibody subtype comprises a heavy chain that has amino acids 1-448 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated, a heavy chain that has amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
2. The method of claim 1 , wherein the antibody subtype is provided in a pharmaceutical composition.
3. The method of claim 2 , wherein the pharmaceutical composition is administered to an individual with rheumatoid arthritis or juvenile idiopathic arthritis for the treatment of the rheumatoid arthritis or juvenile idiopathic arthritis.
4. The method of claim 3 , wherein the individual has rheumatoid arthritis.
5. The method of claim 3 , wherein the individual has juvenile idiopathic arthritis.
6. The method of claim 5 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
7. The method of claim 1 , wherein the antibody subtype has been produced by a Chinese Hamster Ovary (CHO) cell.
8. A method of inhibiting biological activity of interleukin-6 receptor (IL-6R) with an antibody subtype against IL-6R, comprising contacting IL-6R with the antibody subtype, wherein the antibody subtype comprises two heavy chains that have amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
9. The method of claim 8 , wherein the antibody subtype is provided in a pharmaceutical composition.
10. The method of claim 9 , wherein the pharmaceutical composition is administered to an individual with rheumatoid arthritis or juvenile idiopathic arthritis for the treatment of the rheumatoid arthritis or juvenile idiopathic arthritis.
11. The method of claim 10 , wherein the individual has rheumatoid arthritis.
12. The method of claim 10 , wherein the individual has juvenile idiopathic arthritis.
13. The method of claim 12 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
14. The method of claim 8 , wherein the antibody subtype has been produced by a Chinese Hamster Ovary (CHO) cell.
15. A method of inhibiting biological activity of interleukin-6 receptor (IL-6R) with a first antibody subtype and a second antibody subtype against IL-6R, comprising contacting the IL-6R with the first antibody subtype and the second antibody subtype, wherein the first antibody subtype comprises a heavy chain that has amino acids 1-448 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated, a heavy chain that has amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2, and wherein the second antibody subtype comprises two heavy chains that have amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
16. The method of claim 15 , wherein the first antibody subtype and the second antibody subtype are provided in a pharmaceutical composition.
17. The method of claim 16 , wherein the pharmaceutical composition is administered to an individual with rheumatoid arthritis or juvenile idiopathic arthritis for the treatment of the rheumatoid arthritis or juvenile idiopathic arthritis.
18. The method of claim 17 , wherein the individual has rheumatoid arthritis.
19. The method of claim 17 , wherein the individual has juvenile idiopathic arthritis.
20. The method of claim 19 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
21. The method of claim 15 , wherein the first antibody subtype and the second antibody subtype have been produced by a Chinese Hamster Ovary (CHO) cell.
22. A method for the treatment of rheumatoid arthritis or juvenile idiopathic arthritis in an individual, comprising administering to the individual a pharmaceutical composition comprising an antibody subtype, wherein the antibody subtype comprises a heavy chain that has amino acids 1-448 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated, a heavy chain that has amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
23. The method of claim 22 , wherein the treatment is for rheumatoid arthritis.
24. The method of claim 22 , wherein the treatment is for juvenile idiopathic arthritis.
25. The method of claim 24 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
26. The method of claim 22 , wherein the antibody subtype has been produced by a Chinese Hamster Ovary (CHO) cell.
27. A method for the treatment of rheumatoid arthritis or juvenile idiopathic arthritis in an individual, comprising administering to the individual a pharmaceutical composition comprising an antibody subtype, wherein the antibody subtype comprises two heavy chains that have amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
28. The method of claim 27 , wherein the treatment is for rheumatoid arthritis.
29. The method of claim 27 , wherein the treatment is for juvenile idiopathic arthritis.
30. The method of claim 29 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
31. The method of claim 27 , wherein the antibody subtype has been produced by a Chinese Hamster Ovary (CHO) cell.
32. A method for the treatment of rheumatoid arthritis or juvenile idiopathic arthritis in an individual, comprising administering to the individual a pharmaceutical composition comprising a first antibody subtype and a second antibody subtype, wherein the first antibody subtype comprises a heavy chain that has amino acids 1-448 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated, a heavy chain that has amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2, and wherein the second antibody subtype comprises two heavy chains that have amino acids 1-447 of the amino acid sequence set forth in SEQ ID NO: 1 in which an N-terminal glutamine (Gln) is pyroglutamylated and a C-terminal that is Pro-NH 2 , and a light chain that has the amino acid sequence set forth in SEQ ID NO: 2.
33. The method of claim 32 , wherein the treatment is for rheumatoid arthritis.
34. The method of claim 32 , wherein the treatment is for juvenile idiopathic arthritis.
35. The method of claim 34 , wherein the juvenile idiopathic arthritis is systemic idiopathic arthritis.
36. The method of claim 32 , wherein the first antibody subtype and the second antibody subtype have been produced by a Chinese Hamster Ovary (CHO) cell.