Lipolytic enzyme LIP1
The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.
1. A substantially pure polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 85% amino acid sequence identity with an amino acid sequence set forth in SEQ ID NO.: 3, wherein said polypeptide has esterase activity.
2. The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity with an amino acid sequence set forth in SEQ ID NO.: 3.
3. The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 85% sequence identity with an amino acid sequence set forth in SEQ ID NO.:3 and is encoded by a polynucleotide having a nucleic acid sequence having at least 85% nucleic acid sequence identity with a nucleic acid sequence set forth in SEQ ID NO.:1.
4. The polypeptide of claim 3 , wherein said polynucleotide comprises a nucleic acid sequence having at least 95% nucleic acid sequence identity with a nucleic amino acid sequence set forth in SEQ ID NO.: 1.
5. The polypeptide of claim 3 , wherein said polynucleotide encoding a polypeptide is shown in FIG. 3 .
6. A nucleic acid construct comprising a nucleic acid, which encodes for a polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 85% amino acid sequence identity with an amino acid sequence set forth in SEQ ID NO.: 3, wherein said polypeptide has esterase activity; and wherein the nucleic acid is operably linked to one or more control sequences that direct the production of said polypeptide in a suitable host.
7. A recombinant expression vector comprising the nucleic acid construct of claim 6 .
8. A recombinant host cell comprising the nucleic acid construct of claim 6 .
9. The host cell of claim 8 , wherein said host cell is E. coli.
10. A detergent composition comprising the polypeptide of claim 1 .
11. A method for producing an esterase enzyme comprising
a. transforming a host cell with a nucleic acid encoding an esterase enzyme; said nucleic acid having at least 85% nucleic acid sequence identity with a nucleic acid sequence as set forth in SEQ ID NO.: 1;
b. culturing the host cell under conditions to produce the esterase enzyme and
c. recovering the esterase enzyme.
12. The host cell of claim 8 , wherein said host cell is Trichoderma.
13. The host cell of claim 12 , wherein said host cell is Trichoderma reesei.
14. The host cell of claim 8 , wherein said host cell is Aspergillus.
15. The host cell of claim 14 , wherein said host cell is Aspergillus niger.
16. The host cell of claim 8 , wherein said host cell is Bacillus.
17. The host cell of claim 16 , wherein said host cell is selected from the group consisting of B. licheniformis, B. lentos, B. subtilis, B. amyloliquefaciens, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. coagulans, B. circulans, B. pumilus, B. thuringiensis, B. clausii , and B. megaterium.
18. The host cell of claim 17 , wherein said host cell is B. subtilis.
19. A method for cleaning a fabric or removing spots or degreasing objects from a surface, comprising, contacting the fabric or object with a detergent composition comprising the polypeptide of claim 1 .