Native wharton's jelly stem cells and their purification
Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.
1. A method of purifying noncultured Wharton's Jelly stem cells and culturing mesenchymal stem cells, the method comprising:
(a) digesting an umbilical cord tissue comprising Wharton's Jelly, wherein the digesting comprises mincing the umbilical cord tissue;
(b) subsequently diluting the umbilical cord tissue;
(c) subsequently filtering the umbilical cord tissue to generate a filtrate comprising digested Wharton's Jelly and the noncultured Wharton's Jelly stem cells, wherein undigested umbilical cord tissue is filtered from the filtrate;
(d) sedimenting the noncultured Wharton's Jelly stem cells from the filtrate generated in step (c), thereby separating the noncultured Wharton's Jelly stem cells from the digested Wharton's Jelly;
(e) storing the noncultured Wharton's Jelly stem cells at a temperature below −20° C.; and
(f) culturing mesenchymal stem cells from undigested umbilical cord tissue, wherein the mesenchymal stem cells are cultured in the digested Wharton's Jelly.