BGL6 beta-glucosidase and nucleic acids encoding the same
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.
1. An enzymatic composition comprising: a BGL6 polypeptide having at least 90% sequence identity to the amino acid sequence as set forth in SEQ ID NO:2, an endoglucanase (EG), and a cellobiohydrolase (CBH), wherein the BGL6 polypeptide is from a different species than the EG and/or the CBH.
2. The enzymatic composition of claim 1 , wherein the BGL6 polypeptide is from Trichoderma reesei and the EG is from a species other than Trichoderma reesei.
3. The enzymatic composition of claim 2 , wherein the EG is selected from the group consisting of: EGI, EGII, EGIII, EGIV, and EGV endoglucanase.
4. The enzymatic composition of claim 3 , wherein the EG is an EGIV endoglucanase.
5. The enzymatic composition of claim 1 , wherein the BGL6 polypeptide is from Trichoderma reesei and the CBH is from a species other than Trichoderma reesei.
6. The enzymatic composition of claim 5 , wherein the endoglucanase is selected from the group consisting of: CBHI and CBHII cellobiohydrolase.
7. The enzymatic composition of claim 1 , wherein the enzymatic composition is a fermentation culture derived from a recombinant host cell.
8. The enzymatic composition of claim 7 , wherein the recombinant host cell is a filamentous fungi or yeast cell.
9. The enzymatic composition of claim 8 , wherein the recombinant host cell is a filamentous fungi selected from the group consisting of: Trichoderma reesei, Trichoderma longibrachiatum, Trichoderma viride, Trichoderma koningii; Penicillium sp., Humicola sp., Aspergillus sp., Chrysosporium sp., Fusarium sp., Hypocrea sp., and Emericella sp.
10. A method of producing ethanol comprising:
a) contacting a biomass composition with the enzymatic composition of claim 1 to yield a sugar solution;
b) adding to the sugar solution a fermentative microorganism; and
c) culturing the fermentative microorganism under conditions sufficient to produce ethanol.
11. The method of claim 10 further comprising pretreating the biomass composition prior to the contacting step (a).
12. The method of claim 11 , wherein the pretreating comprises contacting the biomass composition with dilute acid.
13. A method of producing ethanol, said method comprising:
a) contacting a biomass composition with the enzymatic composition of claim 1 and a fermentative microorganism; and
b) culturing the fermentative microorganism under conditions sufficient to produce ethanol.
14. The method of claim 13 further comprising pretreating the biomass composition prior to the contacting step (a).
15. The method of claim 14 , wherein the pretreating comprises contacting the biomass composition with dilute acid.