H3 influenza A virus
The invention provides an isolated H3 equine influenza A virus, as well as methods of preparing and using the virus, and genes or proteins thereof.
1. A method to immunize a mammal against influenza, comprising administering to the mammal an effective amount of a composition comprising an H3 influenza virus comprising a gene segment with sequences for a HA having SEQ ID NO:1 or a HA having at least 98% amino acid sequence identity to SEQ ID NO:1 which HA does not have a valine at position 78 or an asparagine at position 159.
2. The method of claim 1 wherein the mammal is a dog or a horse.
3. A diagnostic method comprising contacting a physiological sample of an animal suspected of containing anti-influenza virus antibodies with an H3 influenza virus comprising a gene segment with sequences for a HA having SEQ ID NO:1 or a HA having at least 98% amino acid sequence identity to SEQ ID NO:1 which HA does not have a valine at position 78 or an asparagine at position 159; and determining whether the sample comprises antibodies specific for the virus.
4. The method of claim 1 wherein the H3 influenza virus comprises at least one of the following gene segments: a gene segment with sequences for a NA having SEQ ID NO:2 or having at least 95% amino acid sequence identity to SEQ ID NO:2, a gene segment with sequences for a PB1 having SEQ ID NO:3 or having at least 95% amino acid sequence identity to SEQ ID NO:3, a gene segment with sequences for a PB2 having SEQ ID NO:4 or having at least 95% amino acid sequence identity to SEQ ID NO:4, a gene segment with sequences for a PA having SEQ ID NO:5 or having at least 95% amino acid sequence identity to SEQ ID NO:5, a gene segment with sequences for a NP having SEQ ID NO:6 or having at least 95% amino acid sequence identity to SEQ ID NO:6, a gene segment with sequences for a M1 having SEQ ID NO:7 or having at least 95% amino acid sequence identity to SEQ ID NO:7, a gene segment with sequences for a M2 having SEQ ID NO:17 or having at least 95% amino acid sequence identity to SEQ ID NO:17, a gene segment with sequences for a NS1 having SEQ ID NO:8 or having at least 95% amino acid sequence identity to SEQ ID NO:8, or a gene segment with sequences for a NS2 having SEQ ID NO:18 or having at least 95% amino acid sequence identity to SEQ ID NO:18.
5. The method of claim 1 wherein the composition further comprises a different influenza virus.
6. The method of claim 1 wherein the H3 influenza virus is an attenuated virus or is a reassortant virus.
7. The method of claim 1 wherein the H3 influenza virus has been altered by chemical, physical or molecular means.
8. The method of claim 1 wherein the composition further comprises an adjuvant or a pharmaceutically acceptable carrier.
9. The method of claim 8 wherein the carrier is suitable for intranasal or intramuscular administration.