Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having endoglucanase activity:
(a) has at least 95% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2, or
(b) is a fragment of the sequence of amino acids 27-409 of SEQ ID NO: 2, wherein the polypeptide has endoglucanase activity.
2. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity is a variant of the polypeptide of amino acids 27-409 of SEQ ID NO: 2, wherein the variant comprises a substitution, deletion, and/or insertion of one or more amino acids.
3. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
4. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
5. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
6. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
7. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 27-409 of SEQ ID NO: 2.
8. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity is a fragment of the sequence of amino acids 27-409 of SEQ ID NO: 2 having endoglucanase activity.
9. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide that hybridizes with the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof under high stringency conditions.
10. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 1 .
11. A method of producing a polypeptide having endoglucanase activity, comprising:
(a) cultivating the host cell of claim 10 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
12. A nucleic acid construct or expression vector comprising a polynucleotide encoding polypeptide with a catalytic domain, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide comprises a sequence having at least 95% sequence identity to the catalytic domain of amino acids 27-332 of SEQ ID NO: 2 and has endoglucanase activity.
13. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide having further comprises a cellulose binding domain.
14. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide is a variant, wherein the variant comprises a substitution, deletion, and/or insertion of one or more amino acids of the catalytic domain of amino acids 27-332 of SEQ ID NO: 2.
15. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide comprises a sequence having at least 97% sequence identity to the catalytic domain of amino acids 27-332 of SEQ ID NO: 2.
16. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide comprises a sequence having at least 98% sequence identity to the catalytic domain of amino acids 27-332 of SEQ ID NO: 2.
17. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide comprises a sequence having at least 99% sequence identity to the catalytic domain of amino acids 27-332 of SEQ ID NO: 2.
18. The nucleic acid construct or expression vector of claim 12 , wherein the polypeptide comprises amino acids 27-332 of SEQ ID NO: 2.
19. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 12 .
20. A method of producing a polypeptide with a catalytic domain, comprising:
(a) cultivating the host cell of claim 19 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.