Human cystathionine beta-synthase variants and methods of production thereof
View Patent ↗Human cystathionine β-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine β-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine β-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.
1. A method to produce a recombinant human cystathionine β-synthase comprising:
a) transfecting a recombinant host cell with a recombinant nucleic acid molecule comprising a first nucleic acid sequence encoding a human cystathionine β-synthase or homologue thereof having human cystathionine β-synthase biological activity, wherein the recombinant nucleic acid molecule comprises a recombinant expression vector operatively linked to the first nucleic acid sequence, the expression vector comprising:
i) a second nucleic acid sequence encoding a fusion segment which is linked to the N-terminus of the first nucleic acid sequence by a linker region that will produce a human cystathionine β-synthase fusion protein comprising the fusion segment when the recombinant nucleic acid molecule is expressed; and
ii) a human rhinovirus 3C protease recognition sequence within the linker region; wherein the 5′ nucleotide of the nucleic acid sequence encoding the N-terminal amino acid residue of the human cystathionine β-synthase or homologue thereof is contiguous with the 3′ nucleotide of the nucleic acid sequence encoding the two amino acid residues that occur immediately C-terminal to the human rhinovirus 3C protease recognition site, such that a human cystathionine β-synthase expressed by the recombinant nucleic acid molecule will contain at its N-terminus the two C terminal amino acid residues of the human rhinovirus 3C protease recognition site;
b) culturing the transfected host cell from (a) under conditions effective to produce the recombinant human cystathionine β-synthase fusion protein;
c) contacting the recombinant human cystathionine β-synthase fusion protein with a human rhinovirus 3C protease to cleave the fusion segment from the recombinant human cystathionine β-synthase; and
d) recovering the recombinant human cystathionine β-synthase as a substantially purified recombinant protein wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having an amino acid sequence having an amino terminal deletion or a carboxyl terminal deletion or both an amino terminal and carboxyl terminal deletion and spanning from a starting position of one of amino acid residues from about 1-83 of SEQ ID NO: 2 at the amino terminus to an ending position of one of amino acid residues 382-550 SEQ ID NO: 2 at the carboxyl terminus.
2. The method of claim 1 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 70% identical to SEQ ID NO: 2.
3. The method of claim 1 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 80% identical to SEQ ID NO: 2.
4. The method of claim 1 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 90% identical to SEQ ID NO: 2.
5. The method of claim 1 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having a carboxyl terminal deletion, wherein the cystathionine β-synthase has an ending amino acid position of about 550, 543, 523, 496, 488, 441, 413, 400 or 382, relative to SEQ ID NO:2.
6. The method of claim 1 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having an amino terminal deletion, wherein the cystathionine β-synthase has an starting amino acid position of about 2, 39, 40, 53, 66, 70, 71 or 84, relative to SEQ ID NO:2.
7. A method for producing a truncated form of human cystathionine β-synthase protein having enzymatic activity and having no more than 2 non-human, non-CBS amino acid residues at the N-terminus, the method comprising:
a) transfecting a recombinant host cell with a recombinant nucleic acid molecule comprising a first nucleic acid sequence encoding a human cystathionine β-synthase or homologue thereof having an amino acid sequence having an amino terminal deletion or a carboxyl terminal deletion or both an amino terminal and carboxyl terminal deletion and spanning from a starting position of one of amino acid residues from about 1-83 of SEQ ID NO: 2 at the amino terminus to an ending position to an ending position of one of amino acid residues 382-550 SEQ ID NO: 2 at the carboxyl terminus and having human cystathionine β-synthase biological activity, wherein the recombinant nucleic acid molecule comprises a recombinant expression vector operatively linked to the first nucleic acid sequence, the expression vector comprising:
i) a second nucleic acid sequence encoding a fusion segment which is linked to the N-terminus of the first nucleic acid sequence by a linker region that will produce a human cystathionine β-synthase fusion protein comprising said fusion segment when the recombinant nucleic acid molecule is expressed; and
ii) a rhinovirus 3C protease recognition sequence within the linker region; wherein the 5′ nucleotide of the nucleic acid sequence encoding the N-terminal amino acid residue of the human cystathionine β-synthase or homologue thereof is contiguous with the 3′ nucleotide of the nucleic acid sequence encoding the two amino acid residues that occur immediately C-terminal to the rhinovirus 3C protease recognition site, such that a human cystathionine β-synthase expressed by the recombinant nucleic acid molecule will contain at its N-terminus the two C-terminal amino acid residues of the rhinovirus 3C protease recognition site;
b) culturing the transfected host cell from (a) under conditions effective to produce the recombinant human cystathionine β-synthase fusion protein; and
c) contacting the recombinant human cystathionine β-synthase fusion protein with a rhinovirus 3C protease to cleave the fusion segment from the recombinant human cystathionine β-synthase to produce the truncated form of human cystathionine β-synthase protein having no more than 2 nonhuman, non-CBS amino acid residues at the N-terminus.
8. The method of claim 7 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 70% identical to SEQ ID NO:2.
9. The method of claim 7 , wherein the nucleic acid sequence encoding a human β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 80% identical to SEQ ID NO:2.
10. The method of claim 7 , wherein the nucleic acid sequence encoding a human β-synthase encodes a homologue of human cystathionine β-synthase that is at least about 90% identical to SEQ ID NO:2.
11. The method of claim 7 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having a carboxyl terminal deletion, wherein the cystathionine β-synthase has an ending amino acid position of about 550, 543, 523, 496, 488, 441, 413, 400 or 382, relative to SEQ ID NO:2.
12. The method of claim 7 , wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having an amino terminal deletion, wherein the cystathionine β-synthase has an starting amino acid position of about 2, 39, 40, 53, 66, 70, 71 or 84, relative to SEQ ID NO:2.