Matrices and media for storage and stabilization of biomolecules
The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.
1. A dry-state matrix comprising:
boric acid or a corresponding salt of boric acid; and
a plasticizer and a hydroxyl radical scavenger, wherein histidine is the plasticizer and the hydroxyl radical scavenger;
wherein the matrix is in a solid or dry form which can be converted to a liquid form by suspending, re-hydrating, or solubilizing the matrix in water.
2. The matrix of claim 1 , further comprising a metal chelator.
3. The matrix of claim 1 , wherein said matrix further comprises a singlet oxygen quencher selected from the group consisting of an alkyl imidazole, an indole, a sulfur-containing amino acid, a phenolic compound, an aromatic acid, azide, tocopherol, vitamin E derivatives, carotene, vitamin A derivatives, and any combination thereof.
4. The matrix of claim 1 , wherein said matrix further comprises a metal chelator selected from the group consisting of EDTA, EGTA, o-phenanthroline, citrate, and any combination thereof.
5. The matrix of claim 1 , wherein said matrix further comprises a hydroperoxide removing agent selected from the group consisting of catalase, pyruvate, glutathione, glutathione peroxidases, and any combination thereof.
6. The matrix of claim 1 , Wherein said matrix is inert with respect to methods of nucleic acid amplification, nucleic acid digestion, and/or protein digestion.
7. The matrix of claim 1 , further comprising a sample comprising biomolecules.
8. A method of storing biomolecules in a dry state, said method comprising:
mixing a sample comprising biomolecules with a dry-state matrix of claim 1 to form a mixture, and
drying said mixture to store said biomolecules in a dry state.
9. The method of claim 8 , wherein said sample is a liquid sample, and wherein said liquid sample is mixed with said dry-state matrix.
10. The method of claim 8 , wherein said biomolecules are stored at about 25° C. to about 72° C.
11. The dry-state matrix of claim 1 , further comprising leucine.
12. The dry-state matrix of claim 11 , further comprising a metal chelator.
13. The dry-state matrix of claim 12 , wherein the metal chelator comprises EDTA.
14. The dry-state matrix of claim 13 , wherein the metal chelator further comprises citrate.
15. The dry-state matrix of claim 1 , wherein the dry-state matrix is paper free.
16. The dry-state matrix of claim 1 , wherein the dry-state matrix is in a tube.
17. The dry-state matrix of claim 1 , wherein the dry-state matrix is substantially free of cellulose.
18. The method of claim 8 , wherein said dry-state matrix further comprises a metal chelator selected from the group consisting of EDTA, EGTA, o-phenanthroline, citrate, and any combination thereof.
19. The method of claim 8 , wherein said dry-state matrix further comprises a hydroperoxide removing agent selected from the group consisting of catalase, pyruvate, glutathione, glutathione peroxidases, and any combination thereof.
20. The dry-state matrix of claim 1 , wherein the matrix is substantially free of a biomolecule.
21. The dry-state matrix of claim 1 , wherein the dry-state matrix is carried by a solid support.
22. The dry-state matrix of claim 21 , wherein the solid support is selected from the group consisting of a cotton swab, a filter paper, or a sponge.