Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.
1. A genetically modified bacterial or fungal organism, the genetically modified organism comprising:
at least one exogenous nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide having at least 90% homology to SEQ ID NO:337 and wherein the peptide has alpha-glucuronidase activity.
2. The genetically modified organism of claim 1 , wherein the at least one exogenous nucleic acid from Alicyclobacillus acidocaldarius is integrated into the host organism's genome.
3. The genetically modified organism of claim 1 , wherein the polypeptide has alpha-glucuronidase activity at or above fifty degrees Celsius.
4. The genetically modified organism of claim 1 , wherein the polypeptide comprises SEQ ID NO:337.
5. The genetically modified organism of claim 1 , wherein the at least one exogenous nucleic acid isolated from Alicyclobacillus acidocaldarius has at least 90% homology to SEQ ID NO:336.
6. The genetically modified organism of claim 1 , wherein the at least one exogenous nucleic acid isolated from Alicyclobacillus acidocaldarius is SEQ ID NO:336.
7. A method for at least partially processing hemicellulose into a product, the method comprising:
contacting a liquid culture medium of the genetically modified organism of claim 1 with hemicellulose, wherein the liquid culture medium comprises the polypeptide having alpha-glucuronidase activity.
8. The method according to claim 7 , wherein the contacting occurs at or above fifty degrees Celsius.
9. A method of producing a product from biomass, the method comprising:
pretreating the biomass with an acid;
enzymatically hydrolyzing cellulose and hemicellulose in the biomass to produce monomeric sugars and concurrently fermenting the monomeric sugars to a product; and
recovering the product,
wherein at least a portion of the hydrolyzing of cellulose and hemicellulose or the fermentation is performed by the genetically modified organism of claim 1 .
10. A method of producing a product from biomass, the method comprising:
pretreating the biomass with an acid;
enzymatically hydrolyzing cellulose and hemicellulose in the biomass to produce monomeric sugars;
fermenting the monomeric sugars to a product; and
recovering the product,
wherein at least a portion of the hydrolyzing of cellulose and hemicellulose or the fermentation is performed by the genetically modified organism according to claim 1 , and
wherein the pretreatment, the hydrolyzing of cellulose and hemicellulose, and the fermentation are performed concurrently.
11. A method of processing biomass, the method comprising:
treating the biomass with an acid and concurrently enzymatically hydrolyzing cellulose and hemicellulose in the biomass to produce monomeric sugars,
wherein at least a portion of the hydrolyzing of cellulose and hemicellulose is performed by the genetically modified organism of claim 1 .