Hyaluronic acid-collagen matrices for dermal filling and volumizing applications
Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.
1. A method of improving an aesthetic quality of a soft tissue of a human subject, the method comprising:
(a) providing adipose tissue;
(b) providing a hydrogel composition comprising:
(i) water; and
(ii) a hyaluronic acid crosslinked with a collagen via a plurality of crosslink units,
wherein at least a portion of the crosslink units comprise an amide bond; and
wherein the hyaluronic acid concentration is from 6 mg/mL to 20 mg/mL;
the collagen concentration is from 3 mg/mL to 12 mg/mL;
the hyaluronic acid:collagen weight ratio is from 1:1 to 7:1; and
the hydrogel has a storage modulus value of between 850 Pa and 5,000 Pa;
(c) contacting the hydrogel composition with the adipose tissue ex vivo to provide a mixture; and
(d) administering the mixture to the soft tissue of the subject;
thereby improving the aesthetic quality of the soft tissue.
2. The method of claim 1 , wherein the collagen is collagen type I, collagen type III, or a combination thereof.
3. The method of claim 1 , wherein the collagen is collagen type I.
4. The method of claim 2 or 3 , wherein the collagen is derived from a human or porcine source.
5. The method of claim 1 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 5,000,000 Da.
6. The method of claim 1 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 3,000,000 Da.
7. The method of claim 1 , wherein the ratio of the hyaluronic acid to the collagen is 1:1 to 2:1.
8. The method of claim 1 , wherein the ratio of the hyaluronic acid to the collagen is 1:1.
9. The method of claim 1 , wherein the hydrogel has a storage modulus value of between 1,000 Pa and 5,000 Pa.
10. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL or 16 mg/mL.
11. The method of claim 1 , wherein the hydrogel has a collagen concentration of 6 mg/mL, 8 mg/mL or 12 mg/mL.
12. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 6 mg/mL.
13. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 12 mg/mL.
14. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 16 mg/mL and a collagen concentration of 8 mg/mL.
15. The method of claim 1 , wherein the administration is intradermal.
16. The method of claim 1 , wherein the administration is subcutaneous.
17. The method of claim 1 , wherein the administration is by injection.
18. A method of improving an aesthetic quality of a soft tissue of a human subject, the method comprising:
(a) administering a hydrogel composition to the soft tissue of the subject, wherein the hydrogel composition comprises:
(i) water; and
(ii) a hyaluronic acid crosslinked with a collagen via a plurality of crosslink units,
wherein at least a portion of the crosslink units comprise an amide bond; and
wherein the hyaluronic acid concentration is from 6 mg/mL to 20 mg/mL;
the collagen concentration is from 3 mg/mL to 12 mg/mL;
the hyaluronic acid:collagen weight ratio is from 1:1 to 7:1; and
the hydrogel has a storage modulus value of between 850 Pa and 5,000 Pa;
and
(b) contacting the hydrogel composition with adipose tissue in vivo;
thereby improving the aesthetic quality of the soft tissue.
19. The method of claim 18 , wherein the collagen is collagen type I, collagen type III, or a combination thereof.
20. The method of claim 18 , wherein the collagen is collagen type I.
21. The method of claim 19 or 20 , wherein the collagen is derived from a human or porcine source.
22. The method of claim 18 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 5,000,000 Da.
23. The method of claim 18 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 3,000,000 Da.
24. The method of claim 18 , wherein the ratio of the hyaluronic acid to the collagen is 1:1 to 2:1.
25. The method of claim 18 , wherein the ratio of the hyaluronic acid to the collagen is 1:1.
26. The method of claim 18 , wherein the hydrogel has a storage modulus value of between 1,000 Pa and 5,000 Pa.
27. The method of claim 18 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL or 16 mg/mL.
28. The method of claim 18 , wherein the hydrogel has a collagen concentration of 6 mg/mL, 8 mg/mL or 12 mg/mL.
29. The method of claim 18 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 6 mg/mL.
30. The method of claim 18 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 12 mg/mL.
31. The method of claim 18 , wherein the hydrogel has a hyaluronic acid concentration of 16 mg/mL and a collagen concentration of 8 mg/mL.
32. The method of claim 18 , wherein the administration is intradermal.
33. The method of claim 18 , wherein the administration is subcutaneous.
34. The method of claim 18 , wherein the administration is by injection.