Granted Patent B2

US 9,932,613 · App. 15/792,446 · Granted Apr 3, 2018

Inventors: Haibin Chen (Beijing, CN); Steven J. Collier (Concord, MA); Jovana Nazor (Milpitas, CA); Joly Sukumaran (Singapore, SG); Derek Smith (Singapore, SG); Jeffrey C. Moore (Westfield, NJ); Gregory Hughes (Scotch Plains, NJ); Jacob Janey (New York, NY); Gjalt W. Huisman (Redwood City, CA); Scott J. Novick (Palo Alto, CA); Nicholas J. Agard (San Francisco, CA); Oscar Alvizo (Fremont, CA); Gregory A. Cope (Menlo Park, CA); Wan Lin Yeo (Singapore, SG); Stefanie Ng Minor (Redwood City, CA)

Assignee: Codexis, Inc.

C12P13/02C12N9/0028C12P13/001C12P13/04C12P13/06C12P17/10C12P17/12C12P17/165C12P17/185C12P17/188C12Y105/01C12Y105/01023C12Y105/01024C12Y105/01028Y02P20/52

Abstract

Claims (22)

1. An engineered polynucleotide encoding an engineered polypeptide having imine reductase activity, wherein said polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 2, and comprises a substitution at a position corresponding to position 280 of SEQ ID NO: 2, wherein the amino acid at position 280 has been replaced with an aliphatic or non-polar residue.

2. The engineered polynucleotide of claim 1 , wherein said polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 2, wherein the amino acid at the position corresponding to position 280 of SEQ ID NO: 2 has been replaced with leucine.

3. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1a) pyruvate,

and substrate compound (2b) butylamine

to product compound (3b), N-2-(butylamino)propanoic acid,

under suitable reaction conditions.

4. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone,

and substrate compound (2a) L-norvaline

to product compound (3c), (S)-2-(cyclohexylamino)pentanoic acid,

under suitable reaction conditions.

5. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone,

and substrate compound (2b) butylamine

to product compound (3d), N-butylcyclohexanamine,

under suitable reaction conditions.

6. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1i),

and substrate compound (2b)

to product compound (3n),

under suitable reaction conditions.

7. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1j),

and substrate compound (2b)

to product compound (3o),

under suitable reaction conditions.

Assignments (1)

Continuity (8)